Abstract: Objective To observe morphological characteristics of Schizophyllum commune on five kind of culture media.Methods Schizophyllum commune was cultured on Sabouraud’s Agar (SDA),Malt Extract Agar (MEA),Patato Glucose Agar (PDA),Corn Meal Agar (CMA) and Czapek’s Agar (CZA) respectively at 37℃ and 27℃ for two weeks.Scanning electron microscopy and DNA sequence analysis were performed after one-week culture.Results White and woolly colonies growed better on SDA,MEA and PDA with positive result of urease test and regative result of cycloheximide tolerance test.Septatus mycelium with branches were found under light microscope,and branched hyphae with clamp connections,short and curved lateral pegs and waterdrop-shape excretion were detected under scanning electron microscope.Schizophyllum commune was identified by sequence analysis of 26S rDNA D1/D2.Conclusions Schizophyllum commune had an unique colonial morphology of hyphomycete with characteristic clamp connections,short lateral spicules and waterdrop-like excretion.

Key words: Schizophyllum commune, morphology, molecular biology, scanning electron microscope