Development and architecture characteristics of Aspergillus biofilm

Abstract

Abstract: Objective To investigate the development and architecture characteristics of Aspergillus biofilm.Methods In vitro biofilms of Aspergillus fumigatus(AF293),Aspergillus flavus(BMU03940),Aspergillus terreus(BMU00802) and Aspergillus niger(BMU04689) formed on the surface of sterile plastic cell culture coverslips in 24-well tissue culture plates by adding 200 μL of 1×105 spores/mL cell suspension to each well.After incubation at 37℃ for various time periods(2,4,8,10,12,16,18,24,48 and 72h),the biofilms were stained with FUN-1 in dark for 45 minites.The forming process and vitality were examined under confocal microscope by 488 or combination with 633 argon ion laser stimulation.Calcofluor White stain was applied to observe cytoplasm production with 405 argon ion laser triggering.Results A.fumigatus conidial adhesion occurred at 4h and conidia began to swell and germinate at 8h.Hyphae intertwined and amonolayer formed at 10 to 12h,followed by network structure forming at 16 to 20h.Then the complex three-dimensional architecture with extracellular matrix aroundhyphae was set up at 24h,with gradual matureation at 48 to 72h.Similar to A.fumigatus,the biofilms of A.flavus,A.terreus and A.nigerhad distinct developmental phases including adhesion,germination,filamentation,monolayer development,proliferation and maturation.Conclusions A.fumigatus,A.flavus,A.terreus and A.niger are able to form typical biofilm with distinct developmental phases and architecture characteristics.

Key words: Aspergillus spp, biofilm, architecture characteristic

CLC Number:

R379.6

LI Li-juan, CHEN Wei, XU Hui, WAN Zhe, LI Ruo-yu, LIU Wei. Development and architecture characteristics of Aspergillus biofilm[J]. Chinese Journal of Mycology, 2011, 6(2): 93-97.

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