Prediction and identification of new HLA-A-* 0201-restricted CD8-+ CTL epitopes from Aspergillusfumigatus antigen Asp f16

Abstract

Abstract: Objective To predict and identify potential HLA-A*0201-restricted CD8+ cytotoxic T lymphocyte(CTL) epitopes in Aspergillus fumigatus antigen Asp f16.Methods A total of 427aa Asp f16 amino acid sequences were scanned using HLA peptide binding prediction software on the target of HLA-A*0201.Bone-marrow-derived dendritic cells(DC) and peptide specific CTLs were prepared in HLA A*0201 transgenic mice.Expressions of MHC class II antigen,CD80,CD86,and CD11c were analyzed by flow cytometry.IFN-γ produced by Asp f16 peptides specific CTLs was detected by ELISPOT assay.Tetramer assays were performed to investigate the affinity of the CD8+ CTLs to the Asp f16 peptides complexed HLA-A*0201.Results Three potential HLA-A*0201 binding 9-mer peptides were selected based on their estimated dissociationhalf-life from MHC class I molecular.DCshighly expressed MHC class II antigen,CD80,CD86 and CD11c.Strong affinity of the TCR to the peptide/MHC complex was demonstrated by tetramer staining.Asp f16 peptides specific CD8+ CTL actively produced IFN-γ after exposure to peptide-loaded DCs.Conclusions Three HLA-A*0201-restricted CD8+ CTL epitopes of Asp f16 successfully identified might be candidates for anti Apergillus fumigatus vaccine designing.

Key words: Aspergillus fumigatus, Asp f16, antigen epitope, dendritic cell, cytotoxic T lymphocyte

CLC Number:

R379.6

ZHAO Zuo-tao, SUN Zheng, WAN Zhe, ZHU Ping, LI Ruo-yu. Prediction and identification of new HLA-A-* 0201-restricted CD8-+ CTL epitopes from Aspergillusfumigatus antigen Asp f16[J]. Chinese Journal of Mycology, 2011, 6(2): 87-92.

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