Study on molecular identification and typing of Trichophytonmentagrophytes complex from the representative regions in China

Abstract

Abstract: Objective To study molecular identification and typing of Trichophyton mentagrophytes complex from the representative regions in China.Methods A total of 47 strains of Trichophyton mentagrophytes(T.mentagrophytes) identified by phynotype characteristic from 8 different regions of China were re-identified by sequence analysis of the rRNA gene internal transcribed spacer regions(ITS) and the D1-D2 domain of the large-subunit rRNA gene(LSU D1-D2),and typed by PCR fingerprinting analysis of Trichophyton mentagrophytes using polymorphic subrepeat loci in the rDNA nontranscribed spacer(NTS).The relationship between type and origin of strains was studied.Results Three strains of Trichophyton tonsurans,6 strains of A.benhamiae,and 38 strains of T.interdigitale were identified.Combining PCR fingerprints from each of the three polymorphic loci produced a total of 28 individual strain profiles.No relationship was observed between profiles and the origin and location of strains.Conclusions Trichophyton interdigitale was the most common species from human beings in China.Sequence analysis of ITS combining LSU D1-D2 arehelpful for identification and the typing method using NTS is rapid,reproducible and discriminatory for fragments interpreting.PCR fingerprint analysis of variable tandem repeat loci in the T.mentagrophytes NTS is valuable for future epidemiological investigations.

Key words: Trichophyton mentagrophytes complex, internal transcribed spacer, D1-D2 domain of the large-subunit rRNA gene, nontranscribed spacer

CLC Number:

R379.2

HU Xiao-ping, WAN Zhe, WANG Xiao-hong, LI Ruo-yu. Study on molecular identification and typing of Trichophytonmentagrophytes complex from the representative regions in China[J]. Chinese Journal of Mycology, 2011, 6(2): 70-76.

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