Study on molecular epidemiology of Cryptococcus neoformans species using microsatellite marker

Abstract

Abstract: Objective To study the molecular epidemiology of clinical isolates of Cryptococcus neoformans in China.Methods Totally 116 clinical strains of Cryptococcus neoformans from 1993 to 2009 were isolated and the epidemiology was analyzed by microsatellite genotyping(STR).Results All the 116 strains of C.neoformans belonged to three microsatelite complexes(MCs),with the majority of MC2(n=103),and eight isolates belonged to an unknown microsatellite complex which was labeled as MC12.Conclusions STR is valuble in mocular epidemiological study on Cryptococcus neoformans.

Key words: Cryptococcus neoformans, microsatellite, epidemilolgy

CLC Number:

R379.5

PAN Wei-hua, LIAO Wan-qing, WEN Hai, ZHAO Jin, Ferry Hagen, Teun Boekhout. Study on molecular epidemiology of Cryptococcus neoformans species using microsatellite marker[J]. Chinese Journal of Mycology, 2011, 6(5): 281-284.

References

[1] Baumung R,Cubric-Curik V,Schwend K,et al.Genetic characterisation and breed assignment in austrian sheep breeds using microsatellite marker information[J].Anim Breed Genet,2006,123 (4):265-271.
[2] De Valk HA,Meis JF,Cuffs IM,et al.Use of a novel panel of nine short tandem repeats for exactand high-resolution fingerprinting of Aspergillus fumigatus isolates[J].J Clin Microhiol,2005,43(6):4112-4120.
[3] Viviani MA,Cogliati M,Esposto MC,et al.Molecular analysis of 311 Cryptococcus neoformans isolates from a 30-month ECMM survey of cryptoccosis in Europe[J].FEMS Yeast Research,2006,6(4):614-619.
[4] Chen JH,Varma A,Diaz MR.Cryptococcus neoformans strains and infection in apparently immunocompetent patients,China[J].Emerg Infect Dis,2008,14(5):755-762.
[5] Meyer W,Aanensen DM,Beekhout T,et al.Consensus multilocus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii[J].Med Mycol,2009,47 (6):561-570.
[6] Hagen F,lllnait-Zaragozi MT,Bartlett KH,et al.In vitro susceptibilities and AFLP genotyping of a worldwide collection of 350 clinical,veterinary and environmental Cryptococcus gattii isolates[J].Antimicrob Agents Chemother,2010,54:In press.DOI:10.l128/AAC.00746-10
[7] Illnait-Zaragozi MT,Martinez-Machin GF,Fernandez-Andreu CM,et al.Microsatellite typing of clinical and environmental Cryptococcus neoformans var.grubii isolates from Cuba shows multiple genetic lineages[J].PLoS ONE,2010,5:e9124.
[8] Bart-Delabesse E,Humbert JF,Delabesse E,et al.Microsatellite markers for typing Aspergillusfumigatus isolates[J].J Clin Microbiol,1998,36:2413-2418.
[9] Barreto de Oliveira MT,Boekhout T,Theelen B,et al.Cryptococcus neoformans shows a remarkable genotypic diversity in Brazil[J].J Clin Microbiol,2004,42(3):1356-1359.
[10] Zhu L,Wu J,Xu B,et al.Cryptococcal meningitis in nonHIV-infeeted patients in a Chinese tertiary care hospital,1997-2007[J].Med Mycol,2010,48:570-579.
[11] Jeremy ND,Thu NH,Anh VD,et al.Cryptococcal meningitis in HIV uninfected patients in Vietnam 1 is due to a distinct AFLPdefined cluster of Cryptococcus neoformans var.grubii[J].J Clin Microbiol,2011[Epub ahead of print] doi:10.l128/JCM.01985-10]

[1]	ZHU Xin-lin, HONG Nan, ZHANG Chao, PAN Wei-hua, CAI Liang-qi, LI Xiao-jing, CHEN Min, LIAO Wan-qing. Genotypic diversity of 11 Cryptococcus neoformans isolates from kidney transplantation patients with cryptococcosis [J]. Chinese Journal of Mycology, 2020, 15(6): 338-343.
[2]	MENG Yun-fang, ZHOU Zhao-jing, YANG Ya-li, ZHAO Jing-yu, ZHANG Chao, FA Zhen-zong, SANG Jun-jun, FANG Wei, LIAO Wan-qing. Effects of polyubiquitin UBI4 downregulation on growth and pathogenesis of Cryptococcus neoformans [J]. Chinese Journal of Mycology, 2015, 10(1): 16-21.
[3]	YANG Ya-li, ZHANG Chao, MENG Yun-fang, FA Zhen-zong, ZHOU Zhao-jing, ZHAO Jing-yu, FANG Wei, LIAO Wan-qing. Construction of knockout strains of The COP9 signalosome subunit 6 (CSN6) in Cryptococcus neoformans [J]. Chinese Journal of Mycology, 2014, 9(6): 321-324,330.
[4]	ZHONG Bin, LI Ping, NIE Shu, ZHU Hong-mei, WEN Hai. Gene expression profilinganalysis of mouse brain microvascular endothelial cells incubated with Cryptococcus neoformans [J]. Chinese Journal of Mycology, 2014, 9(4): 203-206.
[5]	ZHAO Jing-yu, FA Zhen-zong, FANG Wei, MENG Yun-fang, ZHANG Chao, ZHOU Zhao-jing, PAN Wei-hua, LIAO Wan-qing. Application of conditional promoter pCTR4 in Cryptococcal gene expression regulation [J]. Chinese Journal of Mycology, 2014, 9(1): 1-6.
[6]	HUANG Hong-yu, LIU Jing-bo, WEI Yun-yu, YAN Zi-he, HAN Zhi-jun. Up-regulation of miR-146a by Cryptococcus neoformans via Dectin-1 [J]. Chinese Journal of Mycology, 2013, 8(6): 325-328.
[7]	ZHOU Jie, PAN Wei-hua, LIAO Wan-qing. The effect of Cryptococcus neoformans capsular GXM on energy metabolism and apoptosis of mouse microglia [J]. Chinese Journal of Mycology, 2013, 8(5): 261-264.
[8]	ZHOU Jie, PAN Wei-hua, LIAO wang-qing. Construction and activity test of animal model of Cryptococcus neoformans biofilm [J]. Chinese Journal of Mycology, 2013, 8(3): 134-136.
[9]	LI Ping, TAN Hong-yue, HU Chan, CHEN Li-hua, HUANG You-ming, ZHONG Bin, ZHU Hong-mei, WEN Hai. Purification of capsular polysaccharide GXM from Cryptococcus neoformans and it's role in regulating MR expression in macrophages [J]. Chinese Journal of Mycology, 2012, (5): 265-268.
[10]	HUANG You-ming, ZHU Hong-mei, WEN Hai. A role of MAPK pathway in the pathogenic mechanism of Cryptcococcus neoformans [J]. Chinese Journal of Mycology, 2012, (5): 304-308.
[11]	XU Nan, XIAO Qin, YAO Wei, CHU Wei-fang, XIAO Xiao-qing, GU Ju-lin, WEN Hai. Effect of mannose receptor of alveolar macrophage on cryptococcal pneumonia in immunodepressive rats [J]. Chinese Journal of Mycology, 2012, 7(3): 146-149,167.
[12]	LI Ping, WEN Hai, ZHU Hong-mei, XU Hong, HU Chan, TAN Hong-yue. Construction and identification of recombinant adenovirus of mannose receptor gene [J]. Chinese Journal of Mycology, 2012, (2): 70-72.
[13]	JIANG Ai-ping, HUANG Jian-qing, LU Wan-ding, XU Ming, WU Jian-hua. Applications of PCR fingerprinting in the study of epidemiology of Candidal vulvovaginitis and balanitis [J]. Chinese Journal of Mycology, 2012, (2): 82-84,115.
[14]	LI Xiu-li, TIAN Yuan, SHI Yu-lin, GU Jun-ying, LI Xiao-jian, LIU Zhi-yu, MA Yue-e, GAO fei, WANG Xiu-xiu. Construction and identification of siRNA expression vector targeting MIS1 gene in Cryptococcus neoformans [J]. Chinese Journal of Mycology, 2012, 7(1): 17-19,23.
[15]	ZHOU Nan, HUANG Chen, PAN Wei-hua, LIAO Wan-qing. Anti-fungal effect of sertraline on Cryptococcus neoformans in vitro and in vivo [J]. Chinese Journal of Mycology, 2011, 6(5): 267-270.