Abstract: Objective To establish a new susceptibility test method for Malassezia furfur.Methods Adding different types and contents of lipid to ATB F2 medium.The MICs of ATCC14521 and clinical strains of M.furfur were measured by ATB Fungus 3.Results After 72 hours incubation,strains of M.furfur grew well in growth control wells.When the concentration of TWEEN 40 was 1% in the wells,strains of M.furfur grew fully,and the results of susceptibility tests was influenced minimally.The different types and contents of lipid could impact the experimental results.Conclusions Using improved ATB Fungus 3 in susceptibility test of M.furfur,the operation was more easy,the results were more easily to read,and the experimental results were repeatability.The growth status of clinical strains in growth control wells was just like that of ATCC14521,and the results were more easily to determine.

Key words: Malassezia furfur, lipid, susceptibility test, ATB Fungus 3