Effect of monomers(pentaeyclic triterpennoids of saikosaponines) from Buplerum kunmingense against itraconazole-resistant Candida albicans strains

Abstract

Abstract: Objective To study the antifungal activity of the monomer Bp3(saikosaponins) isolated from Bupleurum kunmingense with chemical structure of Pentaeyclic triterpennoids.Methods According to the Standard of Clinical and Laboratory Standards Institute(CLSI),the microdilution method and checkerboard microdilution method were used to determine the antifungal activities of Bp3,itraconazole and their combination to itraconazole-resistant C.albicans strains respectively.Results The minimal inhibitory concentrations(MIC) of Bp3 and itraconazole alone were 1.941 μg/mL and 1.008 μg/mL,respectively.In the combination,the MIC of Bp3 and itraconazole were 1.189 μg/mL and 0.346 μg/mL respectively.The geometric mean(MG) of Bp3 and itraconazole were significant reduce in MIC.The interaction analysis showed that synergistic and additive effects were showed in these isolates.Conclusion Comparing with any single agent,the combination of Bp3 and itraconazole was significantly more active to itraconazole-resistant C.albicans strains.

Key words: Buplerum kunmingense, monomers, Candida albicans, itraconazole, in vitro antifungal activity

CLC Number:

R379.4

PIAO Ying-lan, SHEN Liang-liang, YE Yuan-kang, PAN Sheng-li, WU Ji-min. Effect of monomers(pentaeyclic triterpennoids of saikosaponines) from Buplerum kunmingense against itraconazole-resistant Candida albicans strains[J]. Chinese Journal of Mycology, 2012, 7(1): 8-11.

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