Abstract: Objective To clone and construct recombinant adenovirus of mannose receptor(MR) gene from RAW264.7 macrophage cell line.Methods PCR technique and recombination methods were used in cloning and construction recombinant adenovirus of MR gene, Western blot was used to verify the MR protein expressed in HEK293.Results MR gene was amplified from genomic DNA of macrophage and cloned into pShuttle-CMV vector.The linearized pShuttle-CMW plasmid was cotransformed with the pAdEasy-1 vector into the BJ5183 cells,producing the recombination adenovirus which can express the MR protein.Conclusion Successful cloning of MR gene and construction of MR gene expression recombinant adenovirus will be useful for the further research on the functions of MR in Dendritic cells.

Key words: macrophage, MR gene, cloning, recombinant adenovirus, Cryptococcus neoformans