A study on the distribution and the biofilm formation related genes of Candida parapsilosis complex clinically isolated in Guizhou area

Abstract

Abstract: Objective The distribution of infection and drug susceptibility characteristics of Candida parapsilosis complex strains were studied in Guizhou. Detection of the biofilm formation capacity of strains and the expression level of its CDC28 gene were performed. Methods The BACTEC^TM FX and Phoenix-100 instrument were used to identify the strains isolated in there tertiary first class hospitals in Guizhou from January 2017 to December 2019. The Candida parapsilosis complex group was identified by gene sequencing. ATB FUNGUS 3 kit was used to detect drug susceptibility of strains. The Candida parapsilosis biofilms formation capacitywas detected, and the expression levels of their CDC28 genes were detected using qRT-PCR. Results A total of 163 strains of Candida parapsilosis complex were isolated and identified, of which Candida parapsilosis type I, II and III accounted for 73.0%, 6.1% and 20.9% respectively. Urine and blood specimens got the most isolated strains (both 50 strains). Among the ioslates from infected bloodstream, type I, II and III accounted for 54.0%, 10.0% and 36.0% respectively. Three genotype strains were not significantly resistant to antifungal drugs. Among the 17 clinical strains, 16 (94.1%) had biofilm formation ability, of which 2, 4 and 10 strains had strong, general and weak biofilm formation capacity. CDC28 expressed upregulation in 2 strains with strong and 3 strains with general ability. Conclusion The isolated Candida parapsilosis complex was dominated by type I, and the three genotype strains were not obviously resistant to antifungal drugs. Different Candida parapsilosis showed different biofilm formation capacity, and the CDC28 gene might be associated with biofilm formation.

Key words: Candida parapsilosis infection, clinical characteristics, drug susceptibility characteristics, biofilm formation, gene expression

CLC Number:

R379.4

WANG Shun, LI Hong, QU Yujie, LI Tingting, JIN Tingting, HU Fangfang, LUO Zhenhua. A study on the distribution and the biofilm formation related genes of Candida parapsilosis complex clinically isolated in Guizhou area[J]. Chinese Journal of Mycology, 2023, 18(4): 301-309.

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