Transcriptome study of mouse bone marrow-derived macrophages infected by Aspergillus fumigatus spores

Abstract

Abstract: Objective To reveal the important genes related to phagocytosis, immune responses, and clearance of spores in the mouse bone marrow-derived macrophages (BMMs) infected by Aspergillus fumigatus. Methods Mouse primary bone marrow cells were extracted, induced to differentiate into macrophages, and challenged with A1160^c conidia. After infection, the total RNAs of BMMs in the infected group and the control group were extracted and analyzed by RNA-sequencing. Results The primary mouse bone marrow cells were successfully induced to differentiate into BMMs. Compared to untreated control BMMs, the transcription of Toll-like receptor (TLR) Tlr2 and C-type lectin receptors Clec4e and Clec5a were upregulated in conidia-infected BMMs. The NF-κB pathway was also activated through increasing the transcription levels of inflammation related genes such as Il1b, Tnf, Nfkb1, Tnfsf14, Jun, Gsk3b, Tec and Cd40, etc. Meanwhile, the expression of chemokines such as Cxcl2, Cxcl10 and Ccl3 also increased. In addition, the transcription levels of Bax, Nos2, Glul, and Nlrp3, which were related to the generation of reactive oxygen species (ROS), were obviously upregulated. Conclusion To adapt infection of A. fumigatus spores, BMMs activated TLR and C-type lectin receptors, NF-κB pathway, and other immune pathways, exhibiting an elevated immune response and spore clearance potential.

Key words: Aspergillus fumigatus, bone marrow-derived macrophages, transcriptomic sequencing, immune activation, reactive oxygen species

CLC Number:

R379.6

XU Fengjiao, LOU Xinyu, WANG Yuanzhou, HAN Ziyu, ZHONG Guowei. Transcriptome study of mouse bone marrow-derived macrophages infected by Aspergillus fumigatus spores[J]. Chinese Journal of Mycology, 2022, 17(2): 94-98.

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