Protein and RNA analysis of extracellular vesicles of Aspergillus fumigatus

Abstract

Abstract: Objective To analyze the important components in the extracellular vesicles (EVs) of Aspergillus fumigatus to further clarify the pathogenesis of Aspergillus fumigatus. Methods The vesicles of Aspergillus fumigatus were separated by centrifugal method and the morphology was observed by electron microscopy. The size of EVs in solution was analyzed by Marvin nanoparticle tracking analyzer. The processed peptides in the vesicle were analyzed by mass spectrometer. Secondary mass spectrometry data was retrieved using Maxquant (v1.5.2.8). Results The EVs of Aspergillus fumigatus showed obvious bilayer lipid structure under electron microscope. NTA found that the EVs size of Aspergillus fumigatus analysis was mainly concentrated around 130nm. EVs protein contains 9 unstable proteins (15%), the rest was stable proteins, while PI>7 contains 6 proteins. In EVs cytoplasmic proteins was the most, followed by exocrine proteins. However, 25% of EVs could not be located. As predicted by TMPred and GPI, there were 5 proteins located on the bilayer lipid membrane. In RNA, rRNA and tRNA accounted for 59.7% and 29.4% respectively, while miRNA accounted for 0.25%. EVS-miRNA might mainly affect metabolic pathways. Conclusions Aspergillus fumigatus could secrete EVs with a diameter of 130 nm,which containing a variety of proteins, mainly cytoplasmic proteins. EVS-RNA was dominated by rRNA and tRNA, while miRNAs might involve a variety of signaling pathways.

Key words: Aspergillus fumigatus, extracellular vesicles (EVs), proteomics, RNomics

CLC Number:

R379.6

CHEN Chen, HE Bingchan, ZHU Yimin, ZHAO Beilei, CHEN Fei, XU Xiaoyong. Protein and RNA analysis of extracellular vesicles of Aspergillus fumigatus[J]. Chinese Journal of Mycology, 2022, 17(1): 8-11,26.

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