Analysis of functional differentiation between Cyp51A and Cyp51B of Aspergillus fumigatus

Abstract

Abstract: Objective The functional differentiation of Aspergillus fumigatus Cyp51A and Cyp51B was characterized in this study. Method The phylogenetic tree of Cyp51A and Cyp51B was constructed by IQ-tree. The Aspergillus fumigatus Δcyp51A and Δcyp51B mutants were constructed by homologous recombination technology, respectively. The high temperature tolerance and voriconazole susceptibility of both Δcyp51A and Δcyp51B mutants were determined. Moreover, the virulence of Δcyp51A and Δcyp51B mutants was investigated in the Galleria mellonella infection model. Result Phylogenetic analysis revealed that the Cyp51Bs formed a cluster that was separated from Cyp51As. The Δcyp51B mutant showed reduced tolerance to 50 °C when compared to the Δcyp51A and wild-type strain. The Δcyp51A mutant manifested significantly reduced tolerance to voriconazole when compared to that in Δcyp51B mutant. Additionally, there was no statistically significant difference among the Δcyp51A mutant, Δcyp51B mutant, and the wild-type strain. Conclusion The Cyp51A held different functional roles in high temperature tolerance and azole susceptibility of A. fumigatus when compared with Cyp51B.

Key words: Aspergillus fumigatus, Cyp51, functional differentiation

CLC Number:

R379.6

FANG Lingxu, LIANG Hai, WANG Pengyuan, LU Zhongyi, CHEN Fangyan, HAN Li. Analysis of functional differentiation between Cyp51A and Cyp51B of Aspergillus fumigatus[J]. Chinese Journal of Mycology, 2021, 16(6): 373-378.

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