The levels of HMGBl,IL-17 and IL-23 in patients with vulvovaginal candidiasis and its clinical significance

Abstract

Abstract: Objective To investigate the expression and clinical significance of IL-17,IL-23,HMGB1 in vulvovaginal candidiasis. Method Various vulvovaginal candidiasis patients in our hospital were selected as the observation groups,and healthy volunteers were selected as the control group. ELISA was used to detect the expression of HMGB1,IL-17 and IL-23 in serum and vaginal discharge secretion of all subjects,and the relationship between the expression of these indexes and the clinical parameters of VVC was analyzed.Result In this study, 105 VVC patients were divided into mild and moderate VVC group (51 cases), severe VVC group (21 cases) and recurrent vulvovaginal candidiasis(RVVC) group (33 cases). 39 cases with normal physical examination results were selected as control group. In peripheral blood, the levels of HMGB1 in severe VVC group and RVVC group were higher than that in normal group (t=2.971, P=0.008; t=2.365, P=0.005). The levels of IL-17 in mild to moderate and severe VVC group were higher than that in control group (t=2.370, P=0.024; t=2.987, P=0.008), and the levels of IL-23 in mild, moderate and severe VVC group and RVVC group were higher than that in control group (t=2.948, P=0.008; t=2.955, P=0.008; t=2.940, P=0.009); In the vaginal discharge, the levels of IL-17, mild, moderate and severe VVC group and RVVC group were higher than those in control group (t=2.273, P=0.029; t=2.097, P=0.049; t=2.075, P=0.046), the levels of IL-23 in mild to severe VVC group were significantly higher than that in control group (r=2.624, P=0.017). From a correlation analysis between HMGB1, IL-17, IL-23 and clinical parameters of VVC, in peripheral blood, the level of HMGB1 was positively correlated with the expression of IL-17 (r=0.328, P<0.001; r=0.172, P<0.004), and the level of IL-23 was positively correlated with the expression of IL-17 (r=0.689, P<0.001), There was a positive correlation between IL-23 level and IL-17 expression in the vaginal discharge (r=0.268, P<0.001). in peripheral blood, the level of HMGB1 was positively correlated with severity of disease (r=0.286, P=0.001). In the vaginal discharge, the level of IL-17 was positively correlated with the severity of the disease (r=0.273, P=0.001). Conclusion Levels of HMGB1, IL-17 and IL-23 were significantly higher in VVC patients,and they were closely related to disease severity. These indexes may play an important role in the pathogenesis of VVC.

Key words: vulvovaginal candidiasis, HMGB1, IL-17, IL-23

CLC Number:

R519.3

GAO Aili, DAI Xinyue, LI Huaping, WANG Huanli, MA Shaoyin, CHENG Jiaoquan, ZHU Huilan. The levels of HMGBl,IL-17 and IL-23 in patients with vulvovaginal candidiasis and its clinical significance[J]. Chinese Journal of Mycology, 2021, 16(5): 314-318,325.

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