Multicenter retrospective study on antifungal susceptibility and molecular epidemiology of Candida parapsilosis isolated from bloodstream infections in China

Abstract

Abstract: Objectives The present study was to conduct molecular epidemiology and antifungal susceptibilities of C. parapsilosis retrospectively based on multicenter isolates nationwide in China. Methods Atotal of 377 non-duplicated C. parapsilosis isolates from bloodstream infections were collected from unique patients from 42 hospitals across China. Antifungal susceptibility to fluconazole and voriconazole was determined for all isolates using the CLSI M44-A2 disc diffusion method. C. parapsilosis isolates were genotyped using four highly polymorphic microsatellite markers. Results Of the 377 patients, 46.2% were over 60 years old. 26.5%, 23.9%, 21.8% and 12.5% patients were located in surgical ward, medicine ward, general ICU and surgical ICU respectively. Among 377 strains, 24 (6.4%) were resistant to fluconazole and 10 (2.7%) to voriconazole. Microsatellite typing showed that all strains were divided into 172 microsatellite types, 125 MT types only appeared in a single strain; in 28 hospitals, there were 65 cases of which different patients in the same hospital were infected with the same MT type of strains, among which 6 cases indicated different patients were infected with the same MT type of fluconazole resistant strains in the same hospital. Conclusions Here we presented the nationwide molecular epidemiology study of C. parapsilosis causing bloodstream infections in China, and identified several cases of which different patients in the same hospital were infected with the same MT type of strains including antifungal resistant isolates, indicating probable horizontal transmission of C. parapsilosis in hospitals.

Key words: Candida parapsilosis, bloodstream infection, molecular epidemiology, microsatellite typing, antifungal resistance

CLC Number:

R519.3

ZHANG Li, YU Shuying, NING Yating, XIAO Meng, XIAO Yuling, CUI Lanying, CHEN Zhongju, YAN Yan, YE Liyan, XU Yingchun. Multicenter retrospective study on antifungal susceptibility and molecular epidemiology of Candida parapsilosis isolated from bloodstream infections in China[J]. Chinese Journal of Mycology, 2021, 16(5): 289-295.

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