The effect of microevolution on the interaction between Trichosporon assahii and mice macrophage RAW264.7

Abstract

Abstract: Objective To study the effect of microevolution on the interaction between T.asahii and mice macrophage RAW264.7. Methods The original strain (TO) and the evolutional strain (TEVO) were co-cultured with RAW264.7 respectively, then detecting the differences of RAW264.7 phagocytosis and killing the two strains, and the cytotoxicity of the two strains on RAW264.7; analyzing the changes of the cytokines which were secreted by RAW264.7. Results The phagocytic capacity and killing rate of RAW264.7 to TO were significantly higher than that of TEVO. TO had stronger cytotoxicity to RAW264.7 than TEVO. When RAW264.7 and strains were co-cultured with 1:3 or 1:6 for 24h, the secretion of TNF-α and IL-6 by macrophages which co-cultured with TO were higher than that of TEVO, while the secretion of cytokines in TEVO group were higher than that of TO group when co-cultured at 1:9. Conclusion The interaction between the evolutional strain with macrophage was significantly weaker than the original strain which provides a good basis for the long-term coexistence of the evolutional strain in the host.

Key words: microevolution, Trichosporon asahii, innate immunity

CLC Number:

R379.9

PENG Zhuoying, LIAO Yong, YANG Xin, ZHANG Qianyu, YANG Rongya. The effect of microevolution on the interaction between Trichosporon assahii and mice macrophage RAW264.7[J]. Chinese Journal of Mycology, 2021, 16(2): 96-100.

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