Study on the pathogenesis of melanin in Fonsecaea monophora

Abstract

Abstract: Objective To know whether melanin contributes to disease pathogenesis of Fonsecaea monophora. Methods In this study, one albino mutant (CBS125149) was generated from a parent meristematic mutant (CBS122845) of Fonsecaea monophora. Transmission electron microscopy (TEM) profiles showed that melanin in the parent strains appeared as electron-dense granules which located on the cell wall surface. The cell wall fractions from the two different strains were extracted by an alkali-acid method. The different strains or its cell wall fractions were interacted with the activated RAW264.7. Gene expression of i-NOS gene was detected by real time PCR. Expression of nitric oxide(NO) was detected by Griess method. Otherwise, expression results of IL-12, TNF-α, IL-10 was detected by ELISA. Results The pigmented strain and its cell wall fraction could reduce the expression of inducible nitric oxide synthase (i-NOS) gene and inhibit the synthesis of nitric oxide(NO)in vitro (P<0.05). An exacerbated Th2 response and inhibited Th1 response occurred in the interaction between activated RAW264.7 with the pigmented strain or its cell wall fraction. Conclusion Our results suggest that melanin plays an importantrole in fungal escaping from the oxidative burst of macrophages. Melanin causes a negatively Th1 immune response and favors the persistence of the fungal infection.

Key words: melanin, F.monophora, nitric oxide, IL-12, IL-10, TNF-α

CLC Number:

R379.9

Wang Li, Wang Wei. Study on the pathogenesis of melanin in Fonsecaea monophora[J]. Chinese Journal of Mycology, 2021, 16(2): 77-83.

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