Antifungal effect of pyrogallic acid combined with azoles on Candida

Abstract

Abstract: Objective To understand the drug sensitivity of clinical strains of Candida glabrata and the antifungal effect of pyrogallic acid combined with azoles on Candida. Methods One hundred and sixteen clinical isolates of Candida glabrata, 49 strains of Candida albicans,42 strains of Candida tropicalis, 4 strains of Candida krusei, and 13 strains of Candida parapsilosi were collected and tested for drug susceptibility using ATB FUNGUS3 drug sensitivity test strip. At the same time, the antifunal effect of pyrogallic acid combined with azoles on Candida was detected by the checkerboard broth dilution method. Results Of the 116 strains of Candida glabrata, 14.66%(17 strains) were resistant to fluconazole, 22.41%(26 strains) were non-wild-type for itraconazole and 81.03%(94 strains) were non-wild-type for voriconazole. The antifunal effect of pyrogallic acid on 5 kinds of Candida, the MIC value of 46.55% Candida glabrata was 64 μg/mL, the MIC value of 34.69% Candida albicanswas 64 μg/mL, and the MIC value of 59.52% Candida tropicaliswas 64 μg/mL, 25% grams of Candida MIC value of 128 μg/mL, 46.15 Candida nearly MIC value of 128 μg/mL. When azole drugs were combined with pyrogallic acid, 100%, 99.14% and 99.14% of Candida glabrata showed synergistic or additive effects on fluconazole, itraconazole and voriconazole respectively, and there was no significant difference(P>0.05), while Candida albicans,Candida tropicalis, Candida krusei, and Candida parapsilosits all showed irrelevant and antagonistic effects. Compared with the drug alone, 81.03%, 68.1% and 77.59% of Candida glabrata decreased the MIC values of fluconazole, itraconazole, and voriconazole by 2-3 concentration gradients, and there was a statistical difference between drug-resistant and non-resistant groups(P<0.05). Conclusion Candida glabrata hadresistance to azoles, and non-wild-type strain of voriconazole has the highest proportion. When pyrogallic acid was used alone, the antifunal effect of Candida glabrata among the 5 groups of Candida was better, and the antifunal effect of the sensitive group was more significant than the drug-resistant group. The combination of pyrogallic acid and azoles significantly reduced the MIC value of Candida glabrata drugs, and the effect of the drug-resistant group was more significant than that of the non-resistant group, providing experimental evidence for the treatment of clinical Candida glabrata infection by integrated Chinese and western medicine.

Key words: Candida glabrata, pyrogalli cacid, drug sensitivity, synergistic effect

CLC Number:

R978.5

ZHANG Guanyi, YAO Dongting, HU Xiaobo. Antifungal effect of pyrogallic acid combined with azoles on Candida[J]. Chinese Journal of Mycology, 2021, 16(2): 101-105.

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