Analysis of filamentous fungi isolations from clinical laboratories in Hebei province

Chinese Journal of Mycology　2020, Vol. 15 　Issue (4): 206-212.

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Analysis of filamentous fungi isolations from clinical laboratories in Hebei province

ZHAO Ying¹, LI Zhi-rong², ZHAO Jian-hong², LI Fang³, DONG Ai-ying⁴, LIU Jin-lu⁵, GUO Yan-yan⁶, XIE Shou-jun⁷, WU Xiang-kun⁸, GUO Xiu-hua⁹, WANG Jian-sheng¹⁰, ZHANG Jin-yan¹¹, LI Dong-qing¹², WEI Hong-lian¹³, SUN Yan¹⁴, ZHAO Wen-shen¹⁵, REN Hong-tao¹⁶, GUO Ying-hui¹⁷, XIAO Meng¹, XU Ying-chun¹, XU Xiao-lin⁸, WANG Jun-shuang⁸, YANG Wen-hang¹

1. Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences;Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing 100730, China;
2. The Second Hospital of Hebei Medical University, Clinical Laboratory Center of Hebei Province, Heibei 050000, China;
3. Hebei Yanda hopstial, Langfang 065201, China;Chao yang Hospital Affilliated to the Capital University of Medical Science, Beijing 100020, China;
4. North China University of Science and Technology Affiliated Hospital, Tangshan 06300, China;
5. The First Affiated Hospital of Hebei North University, Zhangjiakou 075000, China;
6. Affiated Hospital of Chengde Medical University, Chengde 067000, China;
7. Tangshan Gongren Hospital, Tangshan 063000, China;
8. Hebei Yanda hopstial, Langfang 065201, China;
9. Wu'an First People's Hospital, Wu'an 056300, China;
1
0. Hebei General Hospital, Shijiazhuang 050057, China;
1
1. The Fourth Hospital of Hebei Medical University, Shijiazhuang 050000, China;
1
2. Handan First Hospital, Handan 056002, China;
1
3. The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China;
1
4. Cangzhou Central Hospital, Cangzhou 061001, China;
1
5. Handan Central Hospital, Handan 056001, China;
1
6. Xingtai People's Hospital, Xingtai 054001, China;
1
7. Hebei Children's Hospital, Shijiazhuang 050030, China

Received:2020-02-22 Online:2020-08-28 Published:2020-08-28

Abstract

Abstract:

Objective To investigate the current status of filamentous fungal infections in 15 tertiary teaching hospitals in Hebei province from 2016 to 2017, and to understand the epidemiological characteristics of filamentous fungal infections in Hebei. Methods Filamentous fungi were collected and counted. Sabouraud Dextrose Agar (SDA) and lactophenol cotton blue staining were used for culture and direct microscopic screening and preliminary identification. Vitek MS was used for re-identification of all the strains and ITS and/ or calmodulin sequencing analysis was used to identify the strains which were rare or hard to identify. Results A total of 225 filamentous fungi were collected in 15 tertiary teaching hospitals from 2016 to 2017. Among them, 133 strains of Aspergillus fumigatus (59.11%), 28 strains of Aspergillus flavus /Aspergillus oryzae (12.44%), 18 strains of Aspergillus niger (8.00%), 6 strains of Aspergillus sydowii (2.67%), 6 strains of Aspergillus nidulans (2.67%), and 34 strains of other filamentous fungi (15.11%). Specimen types included sputum (203, 90.22%), ear canal secretion (10, 4.44%), bronchoalveolar lavage fluid (4, 1.78%), and 8 other types (3.56%). The accurate identification rate (148/225) was 65.78%, and the mis-identification rate (77/225) was 34.22%. Conclusion The most common filamentous fungal infection pathogen were Aspergillus spp., mainly Aspergillus fumigatus, Aspergillus flavus and Aspergillus niger. The mis-identification rate of traditional identification methods is higher than 30%. Using mass spectrometry method combined with ITS /CAM sequencing analysis could effectively improve the identification accuracy of filamentous fungi and provide precise etiological basis for clinical treatment.

Key words: filamentous fungi, distribution characteristics, review identification

CLC Number:

R379.2

ZHAO Ying, LI Zhi-rong, ZHAO Jian-hong, LI Fang, DONG Ai-ying, LIU Jin-lu, GUO Yan-yan, XIE Shou-jun, WU Xiang-kun, GUO Xiu-hua, WANG Jian-sheng, ZHANG Jin-yan, LI Dong-qing, WEI Hong-lian, SUN Yan, ZHAO Wen-shen, REN Hong-tao, GUO Ying-hui, XIAO Meng, XU Ying-chun, XU Xiao-lin, WANG Jun-shuang, YANG Wen-hang. Analysis of filamentous fungi isolations from clinical laboratories in Hebei province[J]. Chinese Journal of Mycology, 2020, 15(4): 206-212.

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Analysis of filamentous fungi isolations from clinical laboratories in Hebei province

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