In vitro antifungal susceptibility of 10 Chinese herbal monomers against Sporothrix globosa

Abstract

Abstract:

Objective To investigate the antifungal effects of 10 Chinese herbal monomers against Sporothrix globosa in vitro. Methods The effects of 10 Chinese herbal monomers against 20 strains of S. globosa were determined by micro-broth dilution method with reference to CLSI M38-A2 (3rd). Results The geometric mean(GM) of the minimum inhibitory concentration (MIC) of 10 Chinese herbal monomers ranged from 53.82 μg/mL to>1024 μg/mL. The effects of allicin, berberine, matrine, osthole and paeonol were the best, and the geometric mean were 53.82 μg/mL, 84.45 μg/mL, 103.97 μg/mL, 137.19 μg/mL and 284.05 μg/mL, respectively. Allicin was the most active antifungal against S. globosa, showing the range of MIC was 16-128 μg / mL. The minimum fungicidal concentration (MFC) ranged from 64 μg/mL to >1024 μg/mL. Allicin had the lowest MFC value and GM was 415.87 μg/mL. Conclusion Allicin, berberine, matrine, osthole and paeonol could against S. globosa, and the effects among Chinese herbal monomers were different.

Key words: Sporothrix globosa, Chinese herbal monomers, antifungal susceptibility testing, allicin

CLC Number:

QU Xin-yao, LI Shan-shan, QU Yue, Abdulhakim Salad Nageye, SONG Yang, CUI Yan. In vitro antifungal susceptibility of 10 Chinese herbal monomers against Sporothrix globosa[J]. Chinese Journal of Mycology, 2020, 15(3): 145-149.

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