Abstract:

Objective To compare the susceptibility of Aspergillus fumigatus, Trichophyton mentogrophtes and Cryptococcus neoformans cultured with Sophora alopecuroides total alkaloids, sophocarpine, matrine, sophoridine and oxymatrine. The results will lay a good foundation for further development and utilization of alkaloids from Sophora alopecuroides. Methods Antifungal activity of five kinds of alkaloids against Aspergillus fumigatus, Trichophyton mentogrophtes and Cryptococcus neoformans were detected with both standard broth microdilution method and cylinder-plate assay. Results Sophora alopecuroides total alkaloids, sophocarpine, matrine, sophoridine and oxymatrine were active against Aspergillus fumigatus, Trichophyton mentogrophtes,Cryptococcus neoformans by cylinder-plate method. The MFCs of Sophora alopecuroides total alkaloids, sophocarpine, matrine and sophoridine for Aspergillus fumigatus reached 25.0, 4.25, 5.0, 3.125 mg·mL^-1 respectively. The MFC of oxymatrine for Aspergillus fumigatus was over 500mg·mL^-1.The MFCs of the five types of alkaloids for Trichophyton mentogrophtes were 3.125,2.125,5.0,1.5625,125 mg·mL^-1; the MFCs of Cryptococcus neoformans were 3.125,4.25,10,6.25,1000mg·mL^-1 respectively. Conclusion Sophora alopecuroides total alkaloids, sophocarpine, matrine and sophoridine showed good antifungal activities. But the inhibitory effects of oxymatrine against Aspergillus fumigatus, Trichophyton mentogrophtes and Cryptococcus neoformans were less than that of the other alkaloids.

Key words: Sophora alopecuroides, alkaloids, Aspergillus fumigatus, Trichophyton mentogrophtes, Cryptococcus neoformans