Expression of proinflammatory cytokines in mouse dendritic cells stimulated by Sporothrix globosa

Abstract

Abstract:

Objective To observe the characteristics of the secretion and expression of different inflammatory factors in mouse dendritic cells(DCs) stimulated by the Sporothrix globosa clinical strain in Xinjiang, and to predict the function of the inflammatory factor. Methods The experimental clinical strain was derived from infected lymphatic sporotrichosis, which was configured into different concentrations of fungal suspension (1×10 ⁴ cells/mL to 1×10⁷ cells/mL) to stimulate mouse DC (cell suspension concentration 1×10⁶ cells/mL), cell culture supernatants at concentrations of 6 hours, 24 hours, 48 hours, and 72 hours were collected, and IL-1β, IL-6, IL-4, TNF-α,and IFN-γ were detected by enzyme immunoassay. Results The stimulated DCs secreted IL-1β, IL-6 and TNF-α, and the secretions at different time points were:IL-1β (6 hours:21.26±3.03; 24 hours:24.04±4.25; 48 hours):24.90 ±4.31; 72 hours:27.29 ±6.09), IL-6 (6 hours:44.38 ±3.73; 24 hours:101.72 ±12.28; 48 hours:133.10 ±8.67; 72 hours:180.38 ±13.84), TNF-α (6 hours:860.36 ±20.64; 24 hours:356.03 ±11.46; 48 hours:457.43 ±17.39; 72 hours:1454.53 ±19.46), but no secretory expression was observed for IFN-γ and IL-4.The secretion levels of IL-1β and IL-6 increased gradually with time and dose, but the release of TNF-α was irregularly expressed. Conclusion DCs are involved in the innate immune response of Sporothrix globosa infection. The key inflammatory factors secreted are IL-1β, IL-6, and TNF-α, and the expression levels are TNF-α > IL-6 > IL-1β. The secretion levels of IL-1β and IL-6 are gradually increased in a time- and dose-dependent manner.

Key words: sporotrichosis, Sporothrix globosa, dendritic cells, innate immunity

CLC Number:

R379.9

WANG Xiao-dong, Dlinuer·Taximaimaiti, Dlidaer·Dlixiati, Hadiliya·Hasimu, Paride Abliz. Expression of proinflammatory cytokines in mouse dendritic cells stimulated by Sporothrix globosa[J]. Chinese Journal of Mycology, 2019, 14(6): 321-325.

References

[1] de Beer ZW, Harrington TC,Vismer HF, et al. Phylogeny of Ophiostoma stenoceras Sporothrix schenckii complex[J]. Mycologia, 2003,95(3):434-444.
[2] Zhou X, Rodrigues AM, Feng P, et al. Global ITS diversity in the Sporothrix schenckii complex[J].Fungal Divers, 2014, 66(1):153-165.
[3] Tellez MD, Batista-Duharte A, Portuondo D, et al. Sporothrix schenckii complex biology:environment and fungal pathogenicity[J].Microbiology, 2014,160(11):2325-2365.
[4] 李珊山,刘鹤松,郑华,等. 皮肤型孢子丝菌病585例临床分析[J].中华皮肤科杂志,2011,44(3):161-164.
[5] 张萍,帕丽达·阿布利孜,陈宴,等. 发现于新疆的申克孢子丝菌新基因型[J].临床皮肤科杂志,2011,40(5):262-264.
[6] d'Ostiani CF, Del Sero G, Bacci A, et al. Dendritic cells discriminate between yeasts and hyphae of the fungus Candida albicans. Implications for initiation of T helper cell immunity in vitro and in vivo[J].Exp. Med, 2000,191(10):1661-1674.
[7] Bacci A, Montagnoli C, Perruccio K, et al. Dendritic cells pulsed with fungal RNA induce protective immunity to Candida albicans in hematopoietic transplantation[J]. Immunol, 2002, 168(6):2904-2913.
[8] Bozza S, Perruccio k, Montagnoli C, et al. A dendritic cell vaccine against invasive aspergillosis in allogeneic hematopoietic transplantation[J]. Blood, 2003, 102(10):3807-3814.
[9] Uenotsuchi T, Takeuchi S, et al. Differential induction of Th1-prone immunity by human dendritic cells activated with Sporothrix schenckii of cutaneous and visceral origins to determine their different virulence[J]. Int Immunol,2006,18(12):1637-1646.
[10] Dias NM, Oliveira MME,Portela MA, et al. Sporotrichosis caused by Sporothrix mexicana,Portugal[J].Emerg Infect Dis,2011,17(10):1975-1976.
[11] Marimon R, Gene J, Cano J, et al. Sporothrix luriei:a rate fungus from clinical origin[J].Med Mycol,2008,46(6):621-625.
[12] Marimon R, Cano J, Gene J, et al. Sporothrix brasiliensis, S.globosa, and S.mexican, three new Sporothrix species of clinical interest[J].J Clin Microbiol,2007,45(10):3198-3206.
[13] Marimon R, Gene J, Cano J, et al. Molecular phylogeny of Sporothrix schenckii[J].J Clin Microbiol,2006,44(9):3251-3256.
[14] de Meyer EM, de Beer ZW,Summerbell RC, et al. Taxonomy and phylogeny of new wood and soil inhabiting Sporothrix species in the Ophiostoma stenoceras-Sporothrix schenckii complex[J].Mycologia, 2008,100(4):647-661.
[15] Barros MB,Schubach Ade O, do Valle AC, et al. Cat-transmitted sporotrichosis epidemic in Rio de Janeiro, Brazil:description of a series of cases[J]. Clin Infect Dis,2004,38(4):529-535.
[16] Gong J, Zhang M, Wang Y, et al. Population structure and genetic diversity of Sporothrix globosa in China according to 10 novel microsatellite loci[J]. J Med Microbiol, 2019, 68(2):248-254.
[17] Yu X, Wan Z, Zhang Z, et al. Phenotypic and molecular identification of Sporothrix isolates of clinical origin in Northeast China[J]. Mycopathologia, 2013,176(1-2):67-74.
[18] Silva-Vergara ML,Maneira FRZ, Oliveira RM, et al. Multifocal sporotrichosis with meningeal involvement in a patient with AIDS[J]. Med Mycol,2005,43(2):187-190.
[19] Tachibana T, Matsuyama T,Mitsuyama M. Involvement of CD4⁺T cells and macrophages in acquired protection against infection with Sporothrix schenckii in mice[J]. Med Mycol,1999,37(6):397-404.
[20] Freitas FS, deSiqueira-Hoagland B, do Valle AC, et al. Sporotrichosis in HIV-infection patients:report of 21 cases of endemic sporotrichosis in Rio de Janeiro, Brazil[J]. Med Mycol,2012,50(2):170-178.
[21] Carlos IZ,Sgarbi DBG, Angluster J, et al. Detection of cellular immunity with the soluble antigen of the fungus Sporothrix schenckii in the systemic form of the disease[J]. Mycopathologia,1992,117(3):139-144.
[22] Romani L. Immunity to fungal infections[J]. Nat Rev Immunol,2011,11(4):275-288.
[23] Amanda CG, Lucas SF, Francine AM, et al. The NLRP3 inflammasome contributes to host protection during Sporothrix schenckii infection[J].Immunology,2017,151(2):154-166.
[24] Alba-Fierro CA, Perez-Torres A,Toriello C, et al. Molecular components of the Sporothrix shenckii complex that induce immune response[J].Curr Microbiol,2016,73(2):292-300.
[25] Verdan FF, Faleiros JC, Ferreira LG, et al. Dendritic cell are able to differentially recognize Sporothrix schenckii antigens and promote Th1/Th17 response in vitro[J].Immunobioligy,2012,217(8):788-794.
[26] Zhu J, Yamane H, Paul WE, et al.Differentiation of effector CD4 T cell populations(*)[J].Annu Rev Immunol,2010,28:445-489.
[27] Kawai T, Akira S. Toll-like receptors and their crosstalk with other innate receptors in infection and immunity[J]. Immunity,2011,34(5):637-650.