In vitro evaluation of inhibitory effect and mechanism of various lasers on Trichophyton rubrum under different energies

Abstract

Abstract:

Objective To investigate inhibitory effect of various lasers on Trichophyton rubrum under different energies, and explore the possible mechanism. Methods Plates of standard size of T.rubrum colonies were assigned as control or experimental Groups. Experimental group was irradiated by different lasers, which including Q-switched Nd:YAG 532nm laser, Q-switched Nd:YAG 1064nm laser, long pulse width Nd:YAG 1064nm laser and intense pulsed light. The effects of different lasers energies on isolated colonies were assessed. After irradiation, the sizes of colonies were measured at 1^st, 3^rd and 7^th days, the degree of keratinase production was measured by enzyme-linked immunosorbent assay. Results Compared with the control group, low power lasers did not have any inhibitory effect on the growth and keratinase production of Trichophyton rubrum. Q-switched Nd:YAG 532nm laser at 2-4J/cm²,Q-switched Nd:YAG 1064nm laser at 4-8J/cm² and long pulse width Nd:YAG 1064nm laser at 4J/cm² could significantly inhibit the growth of colonies and the production of keratinase. Conclusion Growth of T.rubrum and the production of keratinase could be effectively suppressed by Q-switched Nd:YAG 532nm laser at 2-4J/cm², Q-switched Nd:YAG 1064nm laser at 4-8J/cm² and long pulse width Nd:YAG 1064nm laser at 4J/cm².

Key words: laser, onychomycosis, Trichophyton rubrum

CLC Number:

R379.2

XIONG Ying, CHEN Ting, CHAI Bao, ZENG Fei-peng, SUN Wen-wen, WU Bo. In vitro evaluation of inhibitory effect and mechanism of various lasers on Trichophyton rubrum under different energies[J]. Chinese Journal of Mycology, 2019, 14(5): 292-296.

References

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