Evaluation of the in vitro antifungal activity of the domestic caspofungin against clinical Aspergillus isolates

Abstract

Abstract:

Objective To evaluate the in vitro antifungal activity and consistency of original and domestic caspofungin against clinical Aspergillus isolates. Methods A total of 75 clinical Aspergillus isolates were collected from Peking Union Medical College Hospital. The antifungal susceptibility testing was carried out using the microdilution method recommended by CLSI. Results The MEC values of echinocandins were generally lower than the MIC values of the azoles, while MEC₅₀ and MEC₉₀ of micafungin were lower two dilutions than that of caspofungin. Except for one Aspergillus flavus isolates with MIC of 4 μg/mL, the MICs of remaining 74 Aspergillus isolates to amphotericin B were no more than 2 μg/mL. All the Aspergillus isolates were susceptible to voriconazole and posaconazole, but 16 Aspergillus fumigatus, 2 Aspergillus flavus and 5 Aspergillus niger isolates were resistant to itraconazole. The MEC values of original and domestic caspofungin against 11 Aspergillus isolates were inconsistent, but the MEC values of domestic caspofungin were only lower one dilution than those of original caspofungin. The consistency occurred in the antifungal susceptibility of over 76% of Aspergillus isolates to caspofungin and micafungin between 24h and 48h results. Conclusions Compared with azoles and amphotericin B, caspofungin had good in vitro antifungal activity against Aspergillus isolates. and domestic caspofungin was highly consistent with original caspofungin.

Key words: caspofungin, antifungal susceptibility, Aspergillus, consistency

CLC Number:

YU Shu-ying, ZHANG Li, LI Ying, XU Ying-chun. Evaluation of the in vitro antifungal activity of the domestic caspofungin against clinical Aspergillus isolates[J]. Chinese Journal of Mycology, 2019, 14(3): 141-146.

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