In vitro chessboard detection for anti-Cryptococcus effect of rattlerin and fluconazole

Abstract

Abstract:

Objective To determine whether there is synergistic effect of rattlerin combined with fluconazole against Cryptococcus in vitro by the checkerboard method. Methods According to the microdilution method (M27-A3), the fluconazole and rattlerin single drug susceptibility test[FLZ, RAT] and the Fractional Inhibitory Concentration Index (FICI) were used as the judgment indicators of combined drug susceptibility test[FLZ, RAT]. Results The MIC values of rattlerin to 37 strains of Cryptococcus were 32-256 μg/mL. When combined with fluconazole, FICI was ≤ 0.50, which reduced the MIC value of fluconazole by 2-16 times. Conclusion Rattlerin had a certain antifungal effect on Cryptococcus, and it could synergize with fluconazole to improve the sensitivity of fluconazole to Cryptococcus.

Key words: rattlerin, fluconazole, Cryptococcus, checkerboard method, combined drug sensitivity

CLC Number:

R379.5

LEI Yan, FANG Wei, ZHANG Chao, LIAO Wan-qing. In vitro chessboard detection for anti-Cryptococcus effect of rattlerin and fluconazole[J]. Chinese Journal of Mycology, 2019, 14(2): 90-94.

References

[1] Zhang C, Chen M, Wang G, et al. Pd@Ag Nanosheets in combination with amphotericin B exert a potent anti-cryptococcal fungicidal effect[J]. Plos One, 2016, 11(6):1-18.
[2] 徐莉,黄几华,邹碧姬. 舍曲林联合两性霉素B抗不同基因型隐球菌的效果[J]. 热带医学杂志, 2018, 18(2):156-163.
[3] 代黎,姜远英,张大志. 抗真菌药物增效剂的研究进展[J]. 中国真菌学杂志, 2013, 8(2):116-119.
[4] 谭善伦,张大志,姜远英. 具有协同抗耐药真菌活性的天然小分子化合物研究进展[J]. 药学学报, 2014, 49(8):1097-1104.
[5] Pandey S, Chatterjee A, Jaiswal S, et al. Protein kinase C-delta inhibitor, rottlerin inhibits growth and survival of mycobacteria exclusively through shikimate kinase[J]. Biochem Biophys Res Commun, 2016, 478(2):721-726.
[6] Kulkarni RR, Tupe SG, Gample SP, et al. Antifungal dimeric chalcone derivative kamalachalcone E from Mallotus philippinensis[J]. Nat Prod Res, 2014, 28(4):245-250.
[7] Nyilasi I, Kocsubé S, Krizsán K, et al. Susceptibility of clinically important dermatophytes against statins and different statin-antifungal combinations[J]. Med Mycol, 2014, 52(2):140-148.
[8] 李强国,李听,曹存巍. 两种钙调磷酸酶抑制剂联合常用抗真菌药物对马尔尼菲青霉酵母相体外药敏的研究[J]. 广西医学大学学报, 2015, 32(2):169-172.
[9] 董怀怀,王元花,廖泽彬. 芒果苷协同氟康唑抗耐药白念珠菌作用研究[J]. 中国真菌学杂志, 2017, 12(2):78-85.
[10] 吴锐,刁文琦,宋延君. 以棋盘法测定汉防己甲素与伊曲康唑的体外联合抗烟曲霉作用[J]. 中国人兽共患病学报, 2013, 29(8):748-752.
[11] Joseph M, Spyros P, Emmanuel R, et al. Defining fractional inhibitory concentration index cutoffs for additive interactions based on self-drug additive combinations, Monte Carlo Simulation analysis, and in vitro-in vivo correlation data for antifungal drug combinations against Aspergillus fumigatus[J]. Antimicrob Agents Chemother, 2010, 54(2):602-609.
[12] 廖勇,杨苏腾,丛林. 格尔德霉素和环孢素联合常用抗真菌药对阿萨希毛孢子菌临床株体外药敏研究[J]. 实用皮肤病学杂志, 2018, 11(2):67-70.
[13] Deng S, Zhang C, Seyedmousavi S, et al. Comparison of the in vitro activities of newer triazoles and established antifungal agents against Trichophyton rubrum[J]. Antimicrob Agents Chemother, 2015, 59(7):4312-4314.
[14] Wei F, Zhenzong F, Wanqing L. Epidemiology of Cryptococcus and cryptococcosis in China[J]. Fungal Genet Biol, 2015, 78(2014), 7-15.
[15] 朱良华,李德东,王彦. 深部真菌感染治疗的新思路[J]. 第二军医大学学报, 2012, 33(5):558-561.
[16] 王彦,黄海,李健峰. 真菌耐药的研究进展[J]. 药学服务与研究, 2009, 9(3):166-170.
[17] 申玲,姜远英,曹永兵. 植物成分协同抗真菌作用的研究进展[J]. 中国真菌学杂志, 2013, 8(1):55-60
[18] 朱均昊,韩德忞,李莉. 华东地区新生隐球菌药物敏感性分析[J]. 检验医学, 2016, 31(9):761-764.
[19] Wang T, Liu C, Jia L. The roles of PKCs in regulating autophagy[J]. J Cancer Res Clin Oncol, 2018, 144(12):2303-2311.
[20] Lei L, Li Z, Zhong G. Rottlerin-mediated inhibition of chlamydia trachomatis growth and uptake of sphingolipids is independent of p38-regulated/activated protein kinase (PRAK)[J]. Plos one, 2012, 7(9):1-6.
[21] Song J, Zhou Y, Gong Y, et al. Rottlerin promotes autophagy and apoptosis in gastric cancer cell lines[J]. Mol Med Rep, 2018, 18(3):2905-2913.
[22] Maioli E, Daveri E, Maellaro E, et al. Non-conventional rottlerin anticancer properties[J]. Arch Biochem Biophys, 2018, 645(2018):50-53.
[23] Shivshankar P, Lei L, Wang J, et al. Rottlerin inhibits chlamydial intracellular growth and blocks chlamydial acquisition of sphingolipids from host cells[J]. Appl Environ Microbiol, 2008, 74(4):1243-1249.
[24] Woo SM, Lee WK, Min KJ, et al. Rottlerin induces cyclooxygenase-2 upregulation through an ATF4 and reactive oxygen species-independent pathway in HEI-OC1 cells[J]. Mol Med Rep, 2016, 14(1):845-850.