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Chinese Journal of Mycology 2019, Vol. 14  Issue (2): 70-77.

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Study on the mechanism of NT-89 against Candida albicans based on quantitative proteomics

LIU Yu, YAN Lan, JIANG Yuan-ying   

  1. Military Special Medicine Research Center, Department of Pharmacy, Naval Medical University, Shanghai 200433, China
  • Received:2018-03-14 Online:2019-04-28 Published:2019-04-28

Abstract:

Objective To analyze the differentially expressed proteins after the treatment of anti-fungal compound NT-89 against Candida albicansby quantitative proteomics iTRAQ technology. Methods Total protein and cell wall proteins of C. albicans were extracted with or without NT-89 treatment. The relative abundanceand differential proteins in protein extracts were detected by iTRAQ. Then the function classification of the differential proteins was performed using GO database. Results 295 differential proteins were detected in extract of total protein (TP), and Ywp1p and Pga10p were most significantly down-regulated in total protein. 6 GPI-anchored proteins in cell wall protein (CWP) extract were found significantly down-regulated. Conclusion NT-89 disrupted the structural integrity and function of cell wall in C. albicans. The iTRAQ technology can provide effective reference information for drug mechanism research.

Key words: NT-89, Candida albicans, cell wall, HF-pyridine, GPI-anchored protein, iTRAQ

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