Analysis of three methods for detecting Cryptococcus in blood and cerebrospinal fluid of AIDS patients

Abstract

Abstract:

Objective To compare the positive rates of Cryptococcus in blood and cerebrospinal fluid (CSF) specimens of patients with HIV/AIDS by the methods of fungal culture, ink staining and Lateral immunochromatography(LFA), hoping to provide a reference for clinicians to diagnose the HIV/AIDS combined with Cryptococcus infection. Methods Totally 1 049 blood and CSF specimens of patients with HIV/AIDS in 2017 were collected for detection by the methods of fungal culture, ink staining and LFA. Besides, CSF specimens were stained with ink. SPSS software (version 14.0) was used for data analysis. Results The positive rates of blood culture, LFA, CSF culture and ink staining for 1 049 patients with HIV/AIDS were 2.8% (19/688), 10.96% (115/1049), 9.18% (29/316), 17.99% (59/328), respectively. 201 patients were both tested by LFA in cerebrospinal fluid and blood at the same time, that positive rates were 27.86% and 37.31%, respectively, with a significant difference statistically (P<0.05). 292 patients with HIV/AIDS were examined by ink staining and LFA in cerebrospinal fluid, that positive rates were 19.52% and 23.97%, respectively, with no significant difference statistically (P>0.05). Conclusion The results of three methods showed that the positive rate of LFA was the highest. Compared with the positive rate of LFA in cerebrospinal fluid, the positive rate of blood samples was higher. So LFA is one ideal method for diagnosis of AIDS complicated with Cryptococcus infection.

Key words: HIV/AIDS, Cryptococcus neoformans, lateral immunochromatography, ink stain, culture

CLC Number:

R446.5

CAO Yu-shuo, QIAN Xue-qin, CAI Jin-feng, CHEN Zhi-jin, ZHAO Yang-yang, ZHU Zhao-qin, LU Hong-zhou. Analysis of three methods for detecting Cryptococcus in blood and cerebrospinal fluid of AIDS patients[J]. Chinese Journal of Mycology, 2018, 13(6): 340-344.

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