The mechanism of Sporothrix globosa melanins protect against antifungal drugs

Abstract

Abstract:

Objective To investigate the effects of Sporothrix globosa melanins on four antifungal agents (TRB, FCZ, ICZ and AMB). Methods The agar plate coating method was used to compare the susceptibility of wild-type strain (MEL⁺) and albino mutant (MEL^-) to antifungal drugs; the effect of melanin on the antifungal drug was detected by ultraviolet spectrophotometry. The HPLC was used to analyze the mechanism of melanins against fungal drugs. Results Antifungal drugs susceptibility results showed that the MEL^- survival rate was significantly lower than MEL⁺ at concentrations of 4~16 μg/mL for AMB and 0.06-0.25 μg/mL for ICZ; the results of UV spectrophotometry assay showed that melanin could significantly reduce the concentration of AMB (P<0.01); the chromatogram of HPLC showed that, compared with control group, new peak was appeared after the addition of melanin.Conclusion Sporothrix globosa melanins can protect against AMB and ICZ, and reduce AMB concentration through transforming, probably.

Key words: Sporothrix globosa, melanin, antifungal drug, susceptibility testing, amphotericin B

CLC Number:

R379.9

CUI Yan, LI Shan-shan, HOU Yuan-yuan, SONG Yang, CHEN Rui-li, LI Hong-xia. The mechanism of Sporothrix globosa melanins protect against antifungal drugs[J]. Chinese Journal of Mycology, 2018, 13(4): 193-196,212.

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