Abstract:

Objective To explore the effect of rifapentine and flueonazole on the cell cycle of FLC-resistance Candida albicans in vitro. Methods Candida albicans suspension were cocultured with rifapentine alone or combined with flueonazole for 12 h. A blank control group without drugs was established. The DNA content of Candida albicans in each group was detected by Flow Cytometrya after Propidium iodide (PI) staining. The effects of drugs on cell cycle of FLC-resistance Candida albicans including the rifapentine alone or combined with flueonazole were analyzed. Results Compared with growth control group and single drug group, DNA content decreased in G0/G1 phase (P<0.01) of combination group, but significantly increased in G2/M phase (P<0.01).This showed that cell cycle was inhibited at G2/M phase when rifapentine and fluconazole were conbined. Conclusion The results showed that the combination of rifapentine and fluconazole had a significantly effect on arresting of FLC-resistance Candida albicans'cell cycel and inhibiting its normal division and proliferation.

Key words: Canidia albicans, rifapentine, fluconazole, cell cycle