In vitro study of the 5-aminolevulinic acid photodynamic therapy for Trichophyton tonsurans strain isolated from granuloma

Abstract

Abstract:

Objective To demonstrate the fungicidal effect and action mechanism of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) against Trichophyton tonsurans strain isolated from granuloma. Methods The clinical isolate of Trichophyton tonsurans granuloma was cultured in potato dextrose agar medium to prepare the suspension. After 6 hours of incubation, the production of PpⅨ was observed by fluorescence microscopy. Following PDT (at light energy density of 50, 100, 150, 175, or 200 J/cm²), the growth inhibitory of this T.tonsurans strain was observed by colony cultivation. Results Fluorescence microscopy showed obvious PpⅨ production of brick red fluorescent substance. At the light energy density of 175 and 200 J/cm², the significantly restricted growth of colonies was evidenced by decreased concentration of suspension. The suspension concentrations were (0.53±0.058)×10⁵ CFU/mL and (0.13±0.057)×10⁵ CFU/mL at light energy density of 175 and 200 J/cm² respectively, which were significantly lower than those of control group, single drug group, single light group and other low energy density ALA-PDT group (P<0.05). Conclusion ALA-PDT exerted fungicidal effect against Trichophyton tonsurans granuloma isolate, which might provide a theoretical basis for the clinical treatment of dermatophytes.

Key words: photodynamic therapy, Trichophyton tonsurans, Majocchi granuloma, dermatophytes

CLC Number:

R379.2

WU Qian-qian, SHI Lei, ZHANG Hai-yan, ZHANG Ling-lin, GAO Zhi-qin, YANG Hong, YANG Lian-juan, WANG Xiu-li. In vitro study of the 5-aminolevulinic acid photodynamic therapy for Trichophyton tonsurans strain isolated from granuloma[J]. Chinese Journal of Mycology, 2018, 13(3): 139-143.

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