Evaluation of fungicidal effect of low frequency ultrasound and amphotericin B-loaded nanoparticles on C. albicans biofilm

Abstract

Abstract:

Objective To evaluate the synergistic fungicidal effect of low frequency ultrasound combined with amphotericin B-loaded nanoparticles (AmB-NPs) on C. albicans biofilm in vitro. Method AmB-NPs were prepared by a polylactic-co-glycolic acid (PLGA) double emulsion method. XTT reduction method was performed to evaluate the effect of ultrasound with AmB-NPs on the activity of mature biofilm. The morphology of biofilm was observed by laser scanning confocal microscope. The protease and phospholipase activities of biofilm were detected by UV spectrophotometer. Results The particle size and shape of AmB-NPs were uniform. The biofilm activity of C. albicans decreased significantly after the joint action of ultrasonic irradiation and AmB-NPs compared with the control group (P<0.01). Simultaneously, the biofilm became thin and loose, with significant decreased activities of protease and phospholipase aenzyme. Conclusion Low-frequency ultrasound combined with AmB-NPs showed significant synergistic antifungal effect against C. albicans biofilm in vitro.

Key words: low frequency ultrasound, C.albicans biofilm, amphotericin B, nanoparticles, synergistic fungicidal

CLC Number:

YANG Min, XIE Shuang, DONG Yu, DU Yong-hong, LI Dai-rong. Evaluation of fungicidal effect of low frequency ultrasound and amphotericin B-loaded nanoparticles on C. albicans biofilm[J]. Chinese Journal of Mycology, 2018, 13(3): 134-138.

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