The research of quantitative real-time PCR testing different specimen in diagnosis of talaromycosis

Abstract

Abstract:

Objective To evaluate real-time fluorescence quantitative PCR (qPCR) in determining the load of Talaromyces marneffei in different samples of talaromycosis marneffei (TSM). Methods The loads of Talaromyces marneffei in pathological slices, biopsy tissues from suspected patients of TSM were measured by means of qPCR. Results The average load of Talaromyces marneffei in pathological slices was 3.01×10⁴ copies (1.07×10⁴~7.26×10⁴ copies), the load of Talaromyces marneffei in biopsy tissues was 4.68×10⁵ copies. Conclusion This study showed that qPCR determining the load of Talaromyces marneffei in pathological slices and biopsy tissues could be a useful method in TSM diagnosis.

Key words: talaromycosis marneffei (TSM), qPCR, diagnosis, pathological slices, biopsy tissues

CLC Number:

R519.8

ZHENG Yan-qing, SHI Na-na, CAO Cun-wei. The research of quantitative real-time PCR testing different specimen in diagnosis of talaromycosis[J]. Chinese Journal of Mycology, 2018, 13(2): 71-74.

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