The establishment and application of PCR ITS1-ITS2 genotyping method in the identification of Prototheca strains

Abstract

Abstract:

Objective To establish the Prototheca PCR ITS1-ITS2 genotyping method, and to generate the phylogenetic tree to study the relationship between different types of Prototheca strains.Methods The extracted DNAs of 5 clinical Prototheca isolates were used to amplify ITS1 and ITS2 region through specific primers. Then, the phylogenetic tree was established with MEGA 6.0 software and verified by SSU rDNA sequencing. Results Among the 5 strains, 1 strain was identified as P. zopfii hydrocarbonea, 2 strains were P. wickerhamii. Although the rest two strains couldn't be identified due to the lack of ITS sequences of P. zopfii portoricensis, they were still classified as P. zopfii and in an independent cluster which was different from P. zopfii hydrocarbonea. This result was verified by SSU rDNA sequencing. Conclusions The method of PCR ITS1-ITS2 genotyping can be used as an effective genotyping method for identification and surveillance of Prototheca infection.

Key words: Prototheca, protothecosis, genotype, identification, ITS region

CLC Number:

R379.9

ZHAO Yue, ZHU Wei-wei, LIU Jin-yan, Meng Ling-ning, Wang Yu-ting, LI Wen-jing, XIANG Ming-jie. The establishment and application of PCR ITS1-ITS2 genotyping method in the identification of Prototheca strains[J]. Chinese Journal of Mycology, 2018, 13(1): 4-7.

References

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