Abstract:

Two children consulted our clinic for erythema and itch for 7 days. Physical examination showed erythema on the perineum.Dermoscopy showed red base with yellow-white scales.Based on the direct microscopic examination, culture on Sabouraud dextrose agar and chromogenic candida agar, serum tube assay and DNA extracted PCR-sequencing identification, candidal intertrigo due to C.albicans was made. Using sterile patch to obtain scales from left side of the crissum before medication, the same method to obtain scales from right side of the crissum after first medication, then put two patches in the same culture medium. The side of sterile patch after medication with sterile cotton (the right side of crissum) showed no colony. Then we used modified agar diffusion method to identify good antifungal activity of 3% clioquinol cream. Cliquinol cream was used in the patient after positive results of direct microscopic examination. The skin lesion was obviously improved and no hyphae or spore were found in the microscopic observation. 3% clioquinol cream can be an option of topical antifungal formulations. Samples prepared with fungal fluorescence staining solution made easier to find hyphae or spore. The innovation of laboratory technique was benefit for diagnose and treatment of patient.

Key words: clioquinol, candidal intertrigo, C.albicans, dermoscopy