Detecting mannan-binding lectin and T-lymphocyte subsets for the diagnosis of pulmonary aspergillosis

Abstract

Abstract:

Objective To investigate the role of mannan-binding lectin (MBL) level and changes of T-lymphocyte subsets on the patients with pulmonary aspergillosis.Methods From May 2013 to May 2015,51 patients were enrolled into disease group,52 healthy adults were enrolled into normalcontrol group.The levels of MBL and Galactomannanwere detected by Enzyme-linked Immunosorbent Assay (ELISA).The percentages of CD3⁺CD4⁺T cells,CD3⁺CD8⁺T cells,and the ratio of CD3⁺CD4⁺/CD3⁺CD8⁺T cells were determined by flow cytometer.Results The express of MBL in disease group (197.96±148.16 μg/ml) was significantly higher than that of normal group (120.25±98.65)(P<0.05).The serum galactomannan (GM) level of disease group (0.94±0.77 μg/L) was significantly higher than that of normal group (0.32±0.16 μg/L).The percentages of CD3⁺CD4⁺T cells and CD3⁺CD8⁺T cells were (33.07±7.9)%,(40.32±7.30)% (P<0.05),(33.00±8.29)%,(25.98±6.65)%(P<0.05)in disease group and normal group,respectively.The ratios of CD3⁺CD4⁺/CD3⁺CD8⁺T cells in disease group and normal group were 1.09±0.47,1.68±0.65 (P<0.05),respectively.Conclusion There was significant change of MBL level and the percentages of T-lymphocyte subset in patients with pulmonary aspergillosis compared with control group.Immunotherapy may improve the efficacy of pulmonary aspergillosis.Through the change of T-lymphocyte subset can preliminary assessment the body's immune status in patients,at the same time,providing a theoretical basis for immune enhancement therapy in pulmonary aspergillosis.

Key words: pulmonary aspergillosis, mannan-binding lectin, tlymphocte, immune

CLC Number:

R519.8

YANG Yan, TENT Ge-ling, WU Yan-hua, ZHANG Lin-lin, GE Li-zhen, CHEN Ying, HU Hua. Detecting mannan-binding lectin and T-lymphocyte subsets for the diagnosis of pulmonary aspergillosis[J]. Chinese Journal of Mycology, 2017, 12(6): 328-331,336.

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