Immunophenotype of peripheral lymphocytes in HIV-negative patients with cryptococcal meningitis

Abstract

Abstract:

Objective To find if immunophenotypic features of peripheral lymphocytes was associated with risk of cryptococcal meningitis (CM) in HIV-negative individuals.Methods A total of 31 CM patients treated at a tertiary hospital in Beijing,China were included.They were divided into immunocompromised comorbidity group (n=16) and apparently immunocompetent group (n=15).Twenty-one healthy donors were included as control group.Lymphocyte subsets including B,NK,CD₄⁺T,and CD₈⁺T cells,CD₂₈ expression on T cells,and activation markers (HLA-DR and CD₃₈) on CD₈⁺T cells were determined by flow cytometry and compared among the three groups.Results Compared with healthy donors,immunocompromised comorbidity group had dramatically decreased median B cell (223 vs.56/μL),NK cell (280 vs.86/μL),CD₄⁺T cell (695 vs.218/μL),and CD₈⁺T cell (521 vs.164/μL) counts (P values for all four subsets<0.001).Proportions of activated CD₈⁺T cells were significantly increased in immunocompromised patients than that in healthy control.Except for CD₈⁺T cell counts (527/μL),the median B cell (128/μL,P=0.02),NK cell (128/μL,P=0.002) and CD₄⁺T cell (567/μL,P=0.03) were moderately reduced in the apparently immunocompetent group than those in the healthy donors.The levels of HLA-DR and CD₃₈ on CD₈⁺T cells in the apparently immunocompetent group were similar with the healthy donors.CD₂₈ expression on CD₄⁺T and CD₈⁺T cells did not differ among the three groups.Conclusions The immunophenotype of CM patients with im-munocompromised comorbidity was characterized by generalized decreased B,NK,CD₄⁺T,and CD₈⁺T cells.Mild-to-moderate reduce in B cell,NK cell and CD₄⁺T cell counts were observed in apparently immunocompetent patients.

Key words: cryptococcal meningitis, lymphocyte subsets, CD₄⁺T cell

CLC Number:

R519.4

XIE Jing, GE Ying, QIU Zhi-feng, ZHANG Kai-yu, LIU Zheng-yin, LI Tai-sheng. Immunophenotype of peripheral lymphocytes in HIV-negative patients with cryptococcal meningitis[J]. Chinese Journal of Mycology, 2017, 12(5): 262-267.

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