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Chinese Journal of Mycology 2017, Vol. 12  Issue (5): 257-261.

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Investigation of the prevalence of Enterocytozoon bieneusi,Encephalitozoon intestinalis and Encephalitozoon cuniculi among diarrheal patients in the southwest of China

QIU Lu-yao1,2, PING Jing1,2, LI Wen-dao1,2, DING Song-tao1, LIU Han-deng1   

  1. 1. Center of Basic Medical Experiment, Chongqing Medical University, Chongqing 401331, China;
    2. College of Pediatrics, Chongqing Medical University, Chongqing 401331, China
  • Received:2017-05-24 Online:2017-10-28 Published:2017-10-28

Abstract:

Objective To investigate the prevalence of Enterocytozoon bieneusi,Encephalitozoon intestinalis and Encephalitozoon cuniculi among diarrheal patients in the southwest of China.Methods A total of 124 fecal specimens from patients with diarrhea syndrome were collected.The specimens were stained by modified trichrome (weber) and were examined under microscopy.The extracted DNA samples were evaluated by PCR amplification.Results The prevalence of E.bieneusi was 7.26% (9/124),and that of E.intestinalis was 3.23% (4/124),with E.cuniculi all negative.Conclusion The infection of microsporidia did exist in the southwest of China,and the most common types in this area were E.bieneusi and E.intestinalis.

Key words: microsporidia, diarrheal patients, infection, zoonotic, PCR amplification

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