The role of IL-22 in oral candidiasis which caused by Candida albicans

Abstract

Abstract:

Objective To evaluate the role of IL-22 in oral candidiasis which caused by Candida albicans. Methods We built the models of experimental oral candidiasis in BALB/c mice firstly.Then we used anti-IL-22 antibody to block the immune response.After that we estimate the manifestation of acute oral candidiasis and colonization of C.albicans in the tongue.Histopathological study and the cytokine changes of buccal mucosa were detected either. Results Mice which IL-22 were blocked showed thick pseudomembranous substance on the tongue and the fungal burden reached 1 700 CFU/mL.The normal mice showed thin pseudomembranous substance and the fungal burden was 980 CFU/mL.Histopathological examinations showed hyphae existing on the mucosal surface and invading epithelium accompanied by destruction of epithelium and inflammatory infiltration in the IL-22 blocked mice.Meanwhile,in the normal mice,there were less hyphae existing and rare inflammation.The Real-time PCR showed that compare with wild mice,the fold change of TNF-α,CXCL-9,CXCL-10,CXCL-11 in IL-22 blocked one were 18.1,0.8,1.2 and 0.9 times.Which were 34.5,1.2,6.1and 1.7 times in IL-22 normal mice. Conclusions IL-22 can be protective through enhance the secretion of chemotactic factor in oral candidiasis which caused by C.albicans.

Key words: Oral candidiasis, Candida albicans, IL-22

CLC Number:

R519.3

TAN Jing-wen, HE Wen-jing, GAO Zhi-qin, YANG Hong, ZHANG Chu-guang, LIANG Yu-lin, YANG Lian-juan. The role of IL-22 in oral candidiasis which caused by Candida albicans[J]. Chinese Journal of Mycology, 2017, 12(4): 207-211.

References

[1] Schaller M,Mailhammer R,Grassl G,et al.Infection of human oral epithelia with Candida species induces cytokine expression correlated to the degree of virulence[J].J Invest Dermatol,2002,118(4):652-657.
[2] Villar CC,Dongari-Bagtzoglou A.Immune defence mechanisms and immunoenhancement strategies in oropharyngeal candidiasis[J].Expert Rev Mol Med,2008,10:e29.
[3] Acosta-Rodriguez EV,Rivino L,Geginat J,et al.Surface phenotype and antigenic specificity of human interleukin 17-producing T helper memory cells[J].Nat Immunol,2007,8(6):639-646.
[4] Cua DJ,Tato CM.Innate IL-17-producing cells:the sentinels of the immune system[J].Nat Rev Immunol,2010,10(7):479-489.
[5] Di Lullo G,Marcatti M,Heltai S,et al.Th22 cells increase in poor prognosis multiple myeloma and promote tumor cell growth and survival[J].Oncoimmunology,2015,4(5):e1005460.
[6] PerusinaLanfranca M,Lin Y,Fang J,et al.Biological and pathological activities of interleukin-22[J].J Mol Med (Berl),2016,94(5):523-534.
[7] Zenewicz LA,Yancopoulos GD,Valenzuela DM,et al.Innate and adaptive interleukin-22 protects mice from inflammatory bowel disease[J].Immunity,2008,29(6):947-957.
[8] Sugimoto K,Ogawa A,Mizoguchi E,et al.IL-22 ameliorates intestinal inflammation in a mouse model of ulcerative colitis[J].J Clin Invest,2008,118(2):534-544.
[9] 王乐.小鼠口腔阴道双部位白念珠菌感染模型的构建及不同部位来源白念珠菌的毒力差异研究[D].北京:京协和医学院研究生院,2014:33-36.
[10] Dumoutier L,Louahed J,Renauld JC.Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF),a novel cytokine structurally related to IL-10 and inducible by IL-9[J].J Immunol,2000,164(4):1814-1819.
[11] Li J,Tomkinson KN,Tan XY,et al.Temporal associations between interleukin 22 and the extracellular domains of IL-22R and IL-10R2[J].Int Immunopharmacol,2004,4(5):693-708.
[12] Ciccia F,Guggino G1,Rizzo A,et al.Interleukin (IL)-22 receptor 1 is over-expressed in primary Sjogren's syndrome and Sj gren-associated non-Hodgkin lymphomas and is regulated by IL-18[J].Clin ExpImmunol,2015,181(2):219-229.
[13] Naher L,Kiyoshima T,Kobayashi I,et al.STAT3 signal transduction through interleukin-22 in oral squamous cell carcinoma[J].Int J Oncol,2012,41(5):1577-1586.
[14] Charo IF,Ransohoff RM.The many roles of chemokines and chemokine receptors in inflammation[J].N Engl J Med,2006,354(6):610-621.
[15] Olson TS,Ley K.Chemokines and chemokine receptors in leukocyte trafficking[J].Am J Physiol Regul Integr Comp Physiol,2002,283(1):R7-28.
[16] Singh SP,Zhang HH,Foley JF,et al.Human T cells that are able to produce IL-17 express the chemokine receptor CCR6[J].J Immunol,2008,180(1):214-221.
[17] Cole AM,Ganz T,Liese AM,et al.Cutting edge:IFN-inducible ELR-CXC chemokines display defensin-like antimicrobial activity[J].J Immunol,2001,15:167(2):623-627.
[18] Egesten A,Eliasson M,Johansson HM,et al.The CXC chemokine MIG/CXCL9 is important in innate immunity against Streptococcus pyogenes[J].J Infect Dis,2007,195(5):684-693.
[19] Maerki C,Meuter S,Liebi M,et al.Potent and broad-spectrum antimicrobial activity of CXCL14 suggests an immediate role in skin infections[J].J Immunol,2009,182(1):507-514.
[20] Wolk K,Witte E,Wallace E,et al.IL-22 regulates the expression of genes responsible for antimicrobial defense,cellular differentiation,and mobility in keratinocytes:a potential role in psoriasis[J].Eur J Immunol,2006,36(5):1309-1323.