Comparative evaluation of Sensititre YeastOne colorimetric panel with broth microdilution methods for antifungal susceptibility testing of Trichosporon asahii isolates

Abstract

Abstract:

Objective To assess the utility of a commercially prepared,colorimetric microdilution panel (Sensititre YeastOne) for testing T.asahii isolates. Methods A total of 62 T.asahii isolates against seven antifungal agents were detected simultaneously by both Sensititre YeastOne and broth microdilution method.The broth microdilution method was adopted as reference method.MIC endpoints were read after 24 h and 48 h of incubation. Results Susceptibility results by broth microdilution method:caspofungin and micafungin showed no in vitro antifungal activities against T.asahii,both of their MIC₉₀s were 16 μg/mL.Azoles had good antifungal activities against T.asahii isolates,MIC₉₀ were fluconazole (8 μg/mL),voriconazole (0.125 μg/mL) and itraconazole (0.5 μg/mL) at 24 h incubation.Three isolates (4.8%,3/62) of T.asahii had high MICs (4 μg/mL) against amphotericin B.After 24 h incubation,essential agreement (EA) between broth microdilution method and Sensititre YeastOne in seven agents were fluconazole (93.5%),voriconazole (98.4%),itraconazole (98.4%),amphotericin B (98.4%),5-flucytosine (88.7%),caspofungin(100%),micafungin(100%),respectively;EA between the two methods decreased to 83.9% (amphotericin B) and 67.7% (flucytosine) when MICs were determined at 48 h incubation.For broth microdilution method,poor agreement was observed mainly for amphotericin B (69.4%) and flucytosine (53.2%),while for Sensititre YeastOne,the overall EA of 24 h and 48 h MICs were excellent except for flucytosine (11.3%). Conclusions Azoles had good in vitro antifungal activities against T.asahii isolates.The Sensititre YeastOne appeared to be comparable to the CLSI broth microdilution method in testing T.asahii isolates and could be used as an alternative for susceptibility testing of T.asahii isolates in clinical laboratories.

Key words: T.asahii, antifungal susceptibility, broth microdilution, Sensititre YeastOne

CLC Number:

R379.9

GUO Li-na, YU Shu-ying, WANG He, XU Ying-chun. Comparative evaluation of Sensititre YeastOne colorimetric panel with broth microdilution methods for antifungal susceptibility testing of Trichosporon asahii isolates[J]. Chinese Journal of Mycology, 2017, 12(4): 198-202,220.

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