Analysis of expression of SRB1、CDR1、ERG11 in fluconazole sensitive and resistant Candida albicans strains

Abstract

Abstract:

Objective To investigate the differences in the expression of Candida albicans SRB1 in Candida albicans fluconazol resistant strain and sensitive strain,and to explore the relationship between SRB1 and drug resistance in Candida albicans.Methods Two Candida albicans strains were from a same parent.We used real-time RT-PCR to observe the expression of the target genes of SRB1,CDR1,ERG11 in Candida albicans fluconazole resistant strain CA-16 and sensitive strain CA-3.Result Compared with CA-3,the expression of the mRNA of SRB1 and drug resistance gene CDR1,ERG11 in CA-16 increased.There was significant difference in the expression levels of SRR1、CDR1 and ERG11 (P<0.05).Conclusion The increased expression of SRB1 and the Candida albicans resistance to fluconazole was closely related,and SRB1 might be a new candidate genes associated with drug resistance.

Key words: Candida albicans, drug resistance, gene, PCR, SRB1

CLC Number:

R379.4

GAO Lai-qiang, WANG Hai-ying, WANG Xue-hong, LI Ai-lan, MA Qiang, ZHANG Hong. Analysis of expression of SRB1、CDR1、ERG11 in fluconazole sensitive and resistant Candida albicans strains[J]. Chinese Journal of Mycology, 2017, 12(3): 148-151,155.

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