Comparison of two immunosuppressive agents on the infection and inflammation of experimental invasive pulmonary aspergillosis mice models

Abstract

Abstract:

Objective To establish an experimental immunosuppressed mice model of invasive pulmonary aspergillosis (IPA),to evaluated various parameters of the innate immune response during the progression of IPA.Methods Immunosuppressed mice received the intratracheal administration of A.fumigatus conidia after two immunosuppressive treatments:a corticosteroid (group A) and a cyclophosphamidum agent (group B).We compared host responses 24 h,48 h and 72 h after infection in terms of survival,lung tissue histopathological analysis,fungal burden,pulmonary production of pro-and anti-inflammatory cytokines.Results The group A showed longer survival than group B did (5.33 vs 2.5 d,P<0.05).Group A showed severe bleeding and congestive,and lung tissue granuloma formation;while group B showed visible A.fumigatus hyphae,tissue necrosis 48 h after infection.Through CFUs and A.fumigatus 18sRNA detections,fugal burdens were significantly lower in lungs of corticosteroid-treated mice than those of cyclophosphamidum-treated mice.This difference was also observed in livers and kidneys,indicating that the level of fungal dissemination was higher in group B than that of group A.In group B mice,the IL-10 concentration increased by 48 h after infection and peaked at 72 h (86+15 pg/mL).No TNF-α production was detected.In group A mice,both TNF-α and IL-10 were detected at very high concentrations at 48 h (3 120±393,434±97 pg/mL).IL-1α expression increased 24 h after infection in group A mice and continually increased within the 3 days.But in group B,IL-1α concentrations maintained with low level.In both group A and B,the expressions of IL-1β increased dramatically after infection.Conclusion Establishing the IPA animal models of the mice by corticosteroid and chemotherapy-treated,We found that IPA pathogenesis was difference between the two groups:the inflammation response of lung was severe in corticosteroid-treated mice than those in chemotherapy-treated mice,while chemotherapy-treated mice showing an adverse host response.

Key words: invasive pulmonary aspergillosis, animal model, mice, immunology, pathology

CLC Number:

R519.8

CHEN Yu-bao, CHEN Fei-fei, ZOU Chun-fang, SHAO Hong-tao. Comparison of two immunosuppressive agents on the infection and inflammation of experimental invasive pulmonary aspergillosis mice models[J]. Chinese Journal of Mycology, 2017, 12(3): 135-139.

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