Detection of physiochemical characterization and synthesis pathway of melanin from Fonsecaea monophora

Abstract

Abstract:

Objective To study the melanin physiochemical characterization and melanin synthesis pathway of Fonsecaea monophora.Methods Pure melanin mass was extracted from the F.monophora isolate using the cell wall crude extract method.Then we used Ultravolet (Uv),Fourier transformed infrared (FT-IR) and electron paramagnetic resonance (EPR) spectra assay to evaluate the physiochemical characterization of melanin.Furthermore,we observed the pigment production of the colonies on different mediums (PDA,PDA with L-DOPA,PDA with DOPA-melanin inhibitors and PDA with DHN-melanin inhibitors),and the quantity of melanin produced on different above media were measured using BioTek Eon microplate reader.Results The melanin extracted from F.monophora shared similar physiochemical and spectroscopic properties with the synthetic L-DOPA melanin.The formation of melanin pigment statistically significant increased on the L-DOPA medium,and decreased on the DOPA-melanin inhibitor medium (sodium azide) and DHN-melanin inhibitors medium (phthalide and tricyclazole) compare to their blank contrast medium.Conclusions The melanin produced by F.monophora is mainly DOPA melanin,and it may synthesize simultaneously by DOPA-melanin and DHN-melanin pathway.

Key words: Fonsecaea monophora, melanin, physiochemical characterization, melanin synthesis pathway

CLC Number:

R379.9

CHEN Ying-dan, LU Chun, LI Mei-rong, MA Han, YIN Song-chao, ZHENG Zhi-xin, LI Hai-yi, XI Li-yan, FENG Pei-ying. Detection of physiochemical characterization and synthesis pathway of melanin from Fonsecaea monophora[J]. Chinese Journal of Mycology, 2017, 12(3): 129-134.

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