Abstract:

Objective To observe the antifungal effect of 10 kinds of traditional Chinese herb extracts in vitro.Methods We performed in vitro drug susceptibility testing for ten kinds of traditional Chinese herb extracts, fluconazole and itraconazole against Candida isolates following CLSI M27-A3 guidelines.Results Panax notoginseng saponins (PNS) and ginseng stem-leave saponins (GSLS) were more sensitive to C.albicans.Their 24 h MIC values were 62.5 μg/mL and the 48 h MIC values were 125 μg/mL.PNS and Citric acid were more sensitive to C.krusei at 24 h and their MIC values were both 1.96 μg/mL.PNS was more sensitive to C.krusei at 48 h, the MIC were 31.25 μg/mL.GSLS and Thymolwere more sensitive to C.parapsilosis and their 24 h MICs were both 3.91 μg/mL.GSLS was sensitive to C.parapsilosis at 48 h, the MIC was 15.63 μg/mL.Conclusion PNS, GSLS, Citric acid and Thymol possessed activity against Candida isolates in vitro.There were some differences in antifungal activity of different extracts of Chinese herbs against different kinds of Candida species.The results provide a theoretical basis for further screening of Chinese herb extracts.

Key words: Chinese herb extracts, Candida, drug susceptibility testing