Synergistic effect of mangiferin in combination with fluconazole against fluconazole-resistant Candida albicans

Abstract

Abstract:

Objective To explore the synergistic effect of mangiferin in combination with fluconazole against fluconazole-resistant Candida albicans.Methods The minimal inhibitory concentrations (MIC₈₀) of 22 fluconazole-resistant Candida albicans strains by mangiferin in combination with fluconazole were determined by checkerboard microdilution assay.Time-kill curves and cell growth test were used to investigate the synergistic effect on Candida albcians growth process.The expression of CDR1, CDR2, MDR1 were measured by Real time RT-PCR.Results Mangiferin in combination with fluconazole could inhibit the growth of Candida albicans obviously (FICI<0.5).Mangiferin and fluconazole-treated cells expressed lower level of CDR1 mRNA than the cells grown in the presence of fluconazole or mangiferin alone.Conclusions Mangiferin possessed a synergistic effect with fluconazole against fluconazole-resistant Candida albcians.

Key words: fluconazole-resistant Candida albicans, mangiferin, fluconazole

CLC Number:

R379.4

DONG Huai-huai, WANG Yuan-hua, LIAO Ze-bin, JIANG Yuan-ying, CAO Ying-ying. Synergistic effect of mangiferin in combination with fluconazole against fluconazole-resistant Candida albicans[J]. Chinese Journal of Mycology, 2017, 12(2): 78-82,85.

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