Evaluation of ITS, BenA and CaM in identification of clinical Aspergillus

Abstract

Abstract:

Objective To evaluate the internal transcribed spacer (ITS), β-tubulin gene (BenA) and calmodulin gene (CaM) sequence analysis in identification of clinical Aspergillus.Methods All 169 strains of Aspergillus collected from clinical patients were analyzed by ITS, BenA, CaM sequencing to obtain the species information with reference to the Genbank database.Results The tested strains were identified to species level with identification rates of 52.7% (89/169), 66.3% (112/169), 97.6% (165/169) and to genus level of 47.3% (80/169), 33.7% (57/169), 2.4% (4/169) by ITS, BenA and CaM, respectively.None of the three sequence markers failed in identification of Aspergillus.Conclusion ITS, BenA and CaM can be used to identify clinical Aspergillus.Among them, CaM is the most efficient molecular marker for Aspergillus identification.

Key words: ITS, BenA, CaM, sequence analysis, Aspergillus, identification

CLC Number:

R379.6

LI Ying, XU Ying-chun. Evaluation of ITS, BenA and CaM in identification of clinical Aspergillus[J]. Chinese Journal of Mycology, 2017, 12(2): 74-77.

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