Effects of transforming growth factor on the function of macrophage against Cryptococcus neoformans

Abstract

Abstract:

Objective To investigate the phagocytosis and killing ability of macrophage under TGF-β intervention.Methods Using TGF-β intervene phagocytosis and killing assay of activated macrophages in vitro.Phagocytic index of macrophage and the intracellular growth of Cryptococcus neoformans was observed respectively.Nitric oxide (NO) production was detected using the Griess regent kit, and then comparing the changes of NO production in macrophages under the TGF-β intervention.Results The phagocytic index with the treatment of TGF-β decreased by 43.9%, significantly lower than control group (P<0.05).While the production of NO increased and the intracellular fungal burden significantly declined under the intervention of TGF-β (P<0.05).Conclusion Chemotaxis and phagocytosis of activated macrophage were inhibited under the intervention of TGF-β in vitro, but the NO production increased and killing ability enhanced.

Key words: Cryptococcus neoformans, transforming growth factor, macrophage

CLC Number:

R379.5

LI Ying-Fang, SANG Jun-Jun, LI Juan, JIANG Wei-Wei, LIAO Wan-Qing, PAN Wei-Hua. Effects of transforming growth factor on the function of macrophage against Cryptococcus neoformans[J]. Chinese Journal of Mycology, 2017, 12(2): 65-68.

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