Effects of Aspergillus fumigatus spore on the activation of mast cell

Abstract

Abstract:

Objective To study the effects of Aspergillus fumigatus spore on the activation of human mast cell line HMC-1.Methods Prepared heat-killed Aspergillus fumigatus spore antigen by heating method.HMC-1 cells were co-cultured with heat-killed Aspergillus fumigatus spores whose concentration was 5×10⁵.Microplate spectrophotometer was applied to the β-hexosaminidase release assay which was an index of mast cell degranulation.Enzyme-linked immunosorbent assay (ELISA) was used for released IL-5.The expression levels of IL-5 mRNA was detected by Realtime-PCR.Results Compared with the negative control group,heat-killed Aspergillus fumigatus spores increased β-hexosaminidase release [(11.3%±1.2%) VS (0.5%±0.1%),P<0.05],IL-5 mRNA expression level [(2.47±0.12) VS (0±0.26),P<0.05] and the release of IL-5 [(11.96±1.09) VS (9±1.36),P<0.05] in HMC-1 cells.Conclusion Aspergillus fumigatus spores activates mast cell line HMC-1 resulting in cell degranulation,synthesis and release of IL-5.

Key words: Aspergillus fumigatus, spore, mast cell, degranulation, interleukin-5

CLC Number:

R379.6

CHEN Li-Sha, LI Yan-Ming, TONG Xun-Liang, ZHAO Zuo-Tao, WANG Chen. Effects of Aspergillus fumigatus spore on the activation of mast cell[J]. Chinese Journal of Mycology, 2017, 12(1): 3-7.

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