Butyl alcohol extract of BaiTouWeng decoction induced apoptosis in Candida albicans biofilms

Abstract

Abstract:

Objective To explore the Butyl alcohol extract of Bai Tou Weng decoction effect on apoptosis of Candida albicans biofilms.Methods Using DCFH-DA staining flow cytometry to detect Candida albicans biofilm cells of reactive oxygen species (ROS) level;Using JC-1 staining of mitochondria of Candida albicans biofilm membrane flow cytometry to detect MMP;Phosphatidylserine externalization was recorded by fluorescence microscopic with Annexin V staining at the early stage of apoptosis in C.albicans cells of biofilms;Metacaspase activity was observed by Fluorescence microscopic with FITC-VAD-FMK staining at the early stage of apoptosis in C.albicans cells of biofilms;Observation on Candida albicans biofilm morphology was performed by DAPI nuclear staining and fluorescence microscopy.Results The apoptosis of Candida albicans significantly increased the proportion of more than 128 ug mL-1BAEB treatment,Caspase activity increased,nuclear pyknosis and fragmentation were observed.Intracellular ROS level was significantly increased,mitochondrial membrane potential decreased significantly.Conclusion The Butyl alcohol extract of BaiTou Weng decoction of certain concentrations could induce apoptosis of Candida albicans biofilm cells.

Key words: Butyl alcohol extract of Bai Tou Weng decoction, C.albicans, biofilms, apoptosis

CLC Number:

R379.4

SHI Gao-xiang, WANG Yun-xia, FENG Xin, SHAO Jing, WANG Tian-ming, WANG Chang-zhong. Butyl alcohol extract of BaiTouWeng decoction induced apoptosis in Candida albicans biofilms[J]. Chinese Journal of Mycology, 2017, 12(1): 13-18.

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