Re-evaluation of the traditional Chinese medicines (TCMs) with antifungal activities recorded in ‘Zhong Yao Da Ci Dian’

Abstract

Abstract:

Objective To investigate the antifungal activity of 127 types of the traditional Chinese medicines (TCMs) with the so-called antifungal activities recorded in ‘Zhong Yao Da Ci Dian’ against Candida albicans,Cryptococcus neoformans and Aspergillus fumigatus in vitro.Methods The portion from each TCM by 75% alcohol extracts were determined for antifungal activity against C. albicans, C. neoformans and A. fumigatus by using the standard CLSI M27-A3 and M38-A2 broth microdilution assays,and further to study the antifungal spectrum and inhibition of hyphae.Results The ethanol extracts from 17 TCMs exhibited the inhibitory activity against C. albicans with MICs ranged from 15.625 to 2 000 μg/mL.34 kinds of ethanol extracts from TCMs had the inhibitory effect against C. neoformans.5 ethanol extracts from TCMs had the inhibitory activity of inhibiting A. fumigatus.Conclusion The antifungal activities of TCMs which has been recorded in ‘Zhong Yao Da Ci Dian’ are measured in vitro by the consistent standard method for the first time,providing the experimental basis for investigation of new antifungal agents.

Key words: Zhong Yao Da Ci Dian', antifungal agents, minimal inhibitory concentration, Candida albicans, Cryptococcus neoformans, Aspergillus fumigatus

CLC Number:

R978.5

WU Jing, WANG Liang, CAO Yong-bing, YAN Lan, JIANG Yuan-ying. Re-evaluation of the traditional Chinese medicines (TCMs) with antifungal activities recorded in ‘Zhong Yao Da Ci Dian’[J]. Chinese Journal of Mycology, 2016, 11(6): 348-353.

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