Comparison of novel fluorescent staining and KOH microscopic examination in fungal measured by direct microscopy

Chinese Journal of Mycology　2016, Vol. 11 　Issue (4): 240-242.

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Comparison of novel fluorescent staining and KOH microscopic examination in fungal measured by direct microscopy

HAN De-min, LIU Yuan-zhi, ZHU Jun-hao, LI Li, ZHANG Qiang-qiang

The Lab of Medical Mycology, Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040

Received:2015-07-27 Online:2016-08-28 Published:2016-08-28

Abstract

Abstract:

Objective To explore a reliable and rapid method in direct microscopic examination for fungi using novel fungal fluorescent staining.Methods Samples from 600 clinically suspected cases of fungal infection were screened by fluorescent staining and KOH microscopic examination.Results Fungal elements were detected in 97% of the cases by fluorescent staining and 88.5% by KOH.Conclusions Fluorescent staining is a reliable and effective method for fungi microscopic examination.

Key words: KOH, fungal direct microscopy, fungal fluorescent staining, fungal infections

CLC Number:

R446.5

HAN De-min, LIU Yuan-zhi, ZHU Jun-hao, LI Li, ZHANG Qiang-qiang. Comparison of novel fluorescent staining and KOH microscopic examination in fungal measured by direct microscopy[J]. Chinese Journal of Mycology, 2016, 11(4): 240-242.

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Comparison of novel fluorescent staining and KOH microscopic examination in fungal measured by direct microscopy

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