In vitro antifungal susceptibilities of mycelium phase of Sporothrix globosa in China

Abstract

Abstract:

Objective To investigate the activities of five antifungal agents on mycelia form of Sporothrix globosa and compare the differences in antifungal susceptibilities in different areas of China.Methods Total 100 isolates which were identified as Sporothrix globosa by molecular identification from Chongqing,Beijing and Jilin were subcultured on 2% potato-dextrose agar plates at 25℃ for 7 d.Then according to modified CLSI microdilution method M38-A2,MICS were measured to evaluate the antifungal activities of potassium iodide(KI),itraconazole(ITC),terbinafin(TRB),fluconazole(FLC),amphotericin B(AmB) to these isolates in vitro.Candida parapsilosis ATCC22019 was used as quality control.Results Potassium iodide showed no antifungal activity against any Sporothrix globosa isolates.TRB gave the lowest geometric mean(GM=0.14 μg/mL) MIC.ITC and AmB showed almost the same activity against all strains tested and the GM was 0.79 μg/mL and 0.63 μg/mL respectively.FLC gave the highest GM MIC(45.89 μg/mL).Seven isolates(four from Jilin,two from Chongqing,one from beijing) were found to be itraconazole-resistant,There was no difference in susceptibility according to the geographical source of the isolates(P>0.05).Conclusion Potassium iodide showed no antifungal activities in vitro.TRB was the most active drug,followed by the ITC and AmB.FLC was the least effective drug.There was no difference in antifungal susceptibilities in different areas of China.

Key words: Sporothrix globosa, mycelium phase, antifungal susceptibility test, micro-broth dilution method

CLC Number:

R379.9

HE Yu, HUANG Yi-jin, ZHAO Mi-mi, ZHOU Xun. In vitro antifungal susceptibilities of mycelium phase of Sporothrix globosa in China[J]. Chinese Journal of Mycology, 2016, 11(4): 209-212.

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